采用含混合碱基的引物和探针组的实时逆转录聚合酶链反应对禽源流感病毒的 H5 和 H7 血凝素基因进行广泛检测。
Broad detection of diverse H5 and H7 hemagglutinin genes of avian influenza viruses by real-time reverse transcription-PCR using primer and probe sets containing mixed bases.
机构信息
Research Team for Zoonotic Diseases, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
出版信息
J Clin Microbiol. 2010 Nov;48(11):4275-8. doi: 10.1128/JCM.01264-10. Epub 2010 Sep 22.
Abstract
Real-time reverse transcription-PCR (RT-PCR) was developed for broad detection of diverse H5 and H7 genes in Eurasian and American lineages of avian influenza viruses by using primer and probe sets containing mixed bases. Optimal use of the mixed bases enabled us to minimize sequence mismatches and to broaden the gene detection spectrum without decreasing sensitivity.
摘要
实时逆转录聚合酶链反应 (RT-PCR) 使用包含混合碱基的引物和探针组，针对欧亚和美洲谱系的禽流感病毒进行了广泛的检测。混合碱基的最佳利用使我们能够最小化序列错配，并在不降低灵敏度的情况下拓宽基因检测范围。
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