利用逆转录聚合酶链反应对禽流感病毒的N1至N9神经氨酸酶基因进行亚型分型。
Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.
作者信息
Tsukamoto Kenji, Ashizawa Takayoshi, Nakanishi Koji, Kaji Noriyuki, Suzuki Kotaro, Shishido Makiko, Okamatsu Masatoshi, Mase Masaji
机构信息
Research Team for Zoonotic Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
出版信息
J Clin Microbiol. 2009 Jul;47(7):2301-3. doi: 10.1128/JCM.02366-08. Epub 2009 Apr 29.
Abstract
Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
摘要
用于扩增禽流感病毒N1至N9神经氨酸酶（NA）基因的逆转录聚合酶链反应以亚型特异性方式检测了119个测试的NA基因中的118个（99.2%）。该技术成功地对从鸟类中分离出的所有167株近期禽流感病毒进行了亚型鉴定。通过对所有285个聚合酶链反应产物的序列分析证实了亚型特异性。
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