Tsukamoto Kenji, Ashizawa Takayoshi, Nakanishi Koji, Kaji Noriyuki, Suzuki Kotaro, Shishido Makiko, Okamatsu Masatoshi, Mase Masaji
Research Team for Zoonotic Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
J Clin Microbiol. 2009 Jul;47(7):2301-3. doi: 10.1128/JCM.02366-08. Epub 2009 Apr 29.
Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
用于扩增禽流感病毒N1至N9神经氨酸酶(NA)基因的逆转录聚合酶链反应以亚型特异性方式检测了119个测试的NA基因中的118个(99.2%)。该技术成功地对从鸟类中分离出的所有167株近期禽流感病毒进行了亚型鉴定。通过对所有285个聚合酶链反应产物的序列分析证实了亚型特异性。