[高效液相色谱法同时测定五味子中五种木脂素]

[Simultaneous determination of five lignans in Schisandra chinensis by HPLC].

作者信息

Ding Zuo, Lu Weiwei, Li Hongbo, Fu Junfan, Piao Zhongyun

机构信息

College of Horticulture, Shenyang Agriculture University, Shenyang 110161, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Jul;35(13):1728-30.

PMID:20862967

Abstract

OBJECTIVE

To develop a HPLC quantitative method for determination of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin in Fructus Schisandrae.

METHOD

A symmetry C18 column (4.6 mm x 250 mm, 5 microm) was used with methanol (A) and water (B) as mobile phases, in gradient elution. The gradient program was as follows: 0-36 min, changed from 60% A to 66% A, 36-65 min, to 80% A, 65-70 min, kept for 80% A, 70-75 min, to 100% A. The flow rate was 0.5 mL x min(-1) and detection wavelength was set at 254 nm.

RESULT

The linearities of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin were in the ranges of 0.0214. 160 (r = 0.9999), 0.020-4.000 (r = 0.9999), 0.021-4.240 (r = 0.9999), 0.020-3.960 (r = 0.9999) and 0.021-4.200 (r = 0.9999). The average recoveries were 104.8%, 104.2%, 102.7%, 104.6%, 104.5%, respectively.

CONCLUSION

The method developed in this study was reliable, and can be used for the quality control of the fruits of S. chinensis.

摘要

目的

建立高效液相色谱法测定五味子中五味子醇甲、五味子醇乙、五味子酯甲、脱氧五味子醇和γ-五味子素的含量。

方法

采用Symmetry C18柱（4.6 mm×250 mm，5μm），以甲醇（A）和水（B）为流动相，梯度洗脱。梯度程序如下：0～36 min，A相由60%变为66%；36～65 min，变为80%；65～70 min，保持80%；70～75 min，变为100%。流速为0.5 mL·min-1，检测波长为254 nm。

结果

五味子醇甲、五味子醇乙、五味子酯甲、脱氧五味子醇和γ-五味子素的线性范围分别为0.0214～160（r = 0.9999）、0.020～4.000（r = 0.9999）、0.021～4.240（r = 0.9999）、0.020～3.960（r = 0.9999）和0.021～4.200（r = 0.9999）。平均回收率分别为104.8%、104.2%、102.7%、104.6%、104.5%。