高效液相色谱法测定五味子不同部位的六种木脂素
[HPLC determination of six lignans in different parts of Schisandra chinensis].
作者信息
Ding Pu, Wang Bing, Song Xin, Li Xian-Kuan, Chen Ting, Liu Cong
机构信息
Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(13):2078-81.
OBJECTIVE
To compare the content of six lignans of different parts of Schisandra chinensis.
METHOD
Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-water gradient system as mobile phase. Wave length was 250 nm. The flow rate was 1 mL x min(-1). Column temperature was 30 degrees C.
RESULT
The total lignans content of wild Schisandra chinensis was higher than that of the cultivated varieties. The total lignans content of different parts varied significantly, wherein the root > main branch > side branches > leaf.
CONCLUSION
This method is stable, reliable, can be used for the quality evaluation of different parts of Schisandra.
目的
比较五味子不同部位六种木脂素的含量。
方法
采用安捷伦TC-C18（4.6 mm×250 mm，5微米）色谱柱，以乙腈-水梯度系统为流动相。检测波长为250 nm。流速为1 mL·min⁻¹。柱温为30℃。
结果
野生五味子总木脂素含量高于栽培品种。不同部位总木脂素含量差异显著，其中根＞主枝＞侧枝＞叶。
结论
该方法稳定、可靠，可用于五味子不同部位的质量评价。
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