Wellcome Trust Centre for Cell Biology, and Edinburgh Centre for Systems Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK.
Biochem Soc Trans. 2010 Oct;38(5):1251-6. doi: 10.1042/BST0381251.
The complex processes of mRNA transcription and splicing were traditionally studied in isolation. In vitro studies showed that splicing could occur independently of transcription and the perceived wisdom was that, to a large extent, it probably did. However, there is now abundant evidence for functional interactions between transcription and splicing, with important consequences for splicing regulation. In the present paper, we summarize the evidence that transcription affects splicing and vice versa, and the more recent indications of epigenetic effects on splicing, through chromatin modifications. We end by discussing the potential for a systems biology approach to obtain better insight into how these processes affect each other.
传统上，mRNA 转录和剪接的复杂过程是分别进行研究的。体外研究表明，剪接可以独立于转录发生，而普遍的观点是，在很大程度上，它可能确实如此。然而，现在有大量证据表明转录和剪接之间存在功能相互作用，这对剪接调控有重要影响。在本文中，我们总结了转录影响剪接和反之亦然的证据，以及最近关于染色质修饰对剪接的表观遗传影响的迹象。最后，我们讨论了采用系统生物学方法来更好地了解这些过程如何相互影响的可能性。
