Dipartimento di Patologia e Clinica Veterinaria, Università degli Studi di Sassari, via Vienna, 2, 07100 Sassari, Italy.
Virology. 2010 Nov 25;407(2):352-9. doi: 10.1016/j.virol.2010.08.034. Epub 2010 Sep 21.
Papillomaviruses play an important role in human cancer development, and have been isolated from a number of animal malignancies. However, the association of papillomaviruses with tumors has been poorly investigated in sheep. In this study, a novel ovine Papillomavirus, OaPV3, was cloned from sheep squamous cell carcinoma. Unlike the already known ovine papillomaviruses, belonging to the Delta genus, OaPV3 lacks the E5 open reading frame and maintains the conserved retinoblastoma motif in the E7 gene. OaPV3 infects exclusively epithelial cells, and was found in skin of healthy sheep of geographically separated flocks located in Sardinia (Italy). This new virus is transcriptionally active in tumors and shares low homology with all the other papillomaviruses, establishing a new genus. Taken together, the co-occurrence of OaPV3 and tumors, its cell and tissue tropism, and its gene repertoire, suggests a role for this virus in development of sheep squamous cell carcinoma.
乳头瘤病毒在人类癌症发展中起着重要作用，并已从许多动物恶性肿瘤中分离出来。然而，乳头瘤病毒与绵羊肿瘤的关联尚未得到充分研究。在这项研究中，从绵羊鳞状细胞癌中克隆出一种新型绵羊乳头瘤病毒 OaPV3。与已知的属于三角洲属的绵羊乳头瘤病毒不同，OaPV3 缺乏 E5 开放阅读框，并在 E7 基因中保持保守的视网膜母细胞瘤基序。OaPV3 仅感染上皮细胞，在来自意大利撒丁岛地理位置不同的羊群的健康绵羊皮肤中发现。这种新病毒在肿瘤中具有转录活性，与所有其他乳头瘤病毒的同源性较低，建立了一个新属。综上所述，OaPV3 与肿瘤的共存、其细胞和组织嗜性以及基因库表明该病毒在绵羊鳞状细胞癌的发展中起作用。
