Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Sassari, Italy.
Mediterranean Center for Disease Control, Sassari, Italy.
Vet Res Commun. 2023 Jun;47(2):641-649. doi: 10.1007/s11259-022-10018-5. Epub 2022 Nov 4.
Cutaneous squamous cell carcinoma (cSCC) is a malignant lesion characterized by proliferation and transformation of keratinocytes in the epidermis and infiltrating derma. cSCC is reported in domestic and wild animal species, worldwide. The occurrence and development of cSCC rely on synergic multifactorial conditions, most importantly sunlight exposure and Papillomavirus (PV) infection. In sheep, the development of such lesions represents a threat both to animal welfare and milk production. Ovis aries papillomavirus 3 (OaPV3) is the main cSCC viral determinant and oncogenic properties of viral E6 and E7 proteins were preliminarily investigated. However, E6 and E7 role and mechanisms resulting in cSCC have not been fully clarified, mainly due to the lack specific immunological tools, such as antibodies for in situ detection of ovine papillomavirus. This paper reports the development of specific serological tools for the investigation of OaPV3 pathogenicity, and their preliminary use to screen 4 ovine cSSC formalin-fixed paraffin embedded tissues. Relevance of immunological tools to investigation of viral biological properties and diagnosis are also discussed.
皮肤鳞状细胞癌 (cSCC) 是一种恶性病变，其特征是表皮和浸润真皮的角质形成细胞增殖和转化。cSCC 发生在全球的家养和野生动物物种中。cSCC 的发生和发展依赖于协同的多因素条件，最重要的是阳光暴露和乳头瘤病毒 (PV) 感染。在绵羊中，此类病变的发展对动物福利和牛奶生产构成威胁。绵羊乳头瘤病毒 3 (OaPV3) 是主要的 cSCC 病毒决定因素，病毒 E6 和 E7 蛋白的致癌特性已初步研究。然而，E6 和 E7 导致 cSCC 的作用和机制尚未完全阐明，主要是因为缺乏特异性免疫工具，例如用于原位检测绵羊乳头瘤病毒的抗体。本文报告了用于研究 OaPV3 致病性的特异性血清学工具的开发，及其初步用于筛选 4 份绵羊 cSSC 福尔马林固定石蜡包埋组织。还讨论了免疫工具与病毒生物学特性研究和诊断的相关性。
