School of Health and Bioscience, University of East London, London, United Kingdom.
J Ethnopharmacol. 2011 Jan 7;133(1):116-9. doi: 10.1016/j.jep.2010.09.011. Epub 2010 Sep 21.
Determination of pharmacological activity relevant to wound healing of Bridelia ferruginea leaf, a traditional medicine used to treat wounds in rural Nigeria.
Aqueous and ethanolic leaf extracts were tested against bacterial species of relevance to wound infections: Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. The ethanolic extracts were assessed for their ability to stimulate the growth of human dermal fibroblasts (FS5) and protect against damage induced by hydrogen peroxide. Antioxidant activity was also assessed using the DPPH assay.
Both aqueous and ethanolic extracts had weak antibacterial activity (MIC>470 μg/ml). A significant effect (p<0.001) on the growth of FS5 fibroblasts was observed only at a concentration of 5 μg/ml (28% increase), above which the extracts appeared toxic to the cells. The ethanolic extract offered the highest protection against H(2)O(2) damage to FS5 cells, comparable with catalase (82% at 250 μg/ml). The DPPH assay revealed antioxidant activity of the ethanolic leaf extract with IC(50) 12.5±0.3 μg/ml comparable to l-ascorbic acid (7.3±0.1 μg/ml).
The antibacterial, modest fibroblast stimulation activity and relatively strong antioxidant activity lend some support to the topical use of Bridelia ferruginea leaf for wound-healing in the traditional medicine of South-western Nigeria.
测定用于治疗尼日利亚农村地区伤口的传统药物铁仔叶与伤口愈合相关的药理学活性。
采用与伤口感染相关的细菌种类（金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和铜绿假单胞菌）对水提物和醇提物进行了测试。评估了醇提物对人真皮成纤维细胞（FS5）生长的刺激作用以及对过氧化氢诱导损伤的保护作用。还使用 DPPH 测定法评估了抗氧化活性。
水提物和醇提物均具有较弱的抗菌活性（MIC>470μg/ml）。仅在浓度为 5μg/ml（增加 28%）时才观察到对 FS5 成纤维细胞生长的显著影响（p<0.001），在此浓度之上，提取物对细胞似乎有毒。醇提物对 FS5 细胞中 H2O2 损伤的保护作用最高，与过氧化氢酶相当（250μg/ml 时为 82%）。DPPH 测定法显示，醇提物具有抗氧化活性，IC50 为 12.5±0.3μg/ml，与 l-抗坏血酸（7.3±0.1μg/ml）相当。
铁仔叶具有抗菌、适度刺激成纤维细胞活性和相对较强的抗氧化活性，这为其在尼日利亚西南部传统医学中用于伤口愈合的局部应用提供了一定的依据。
