Graduate Program in Biomedical Sciences, University Center of Herminio Ometto Foundation-FHO, Dr. Maximiliano Baruto Ave, 500. Jardim Universitario, Araras, Sao Paulo, 13607-339, Brazil.
Graduate Program of Pharmaceutical Sciences, Laboratory of Natural Products, University of Vila Velha-UVV, Comissario Jose Dantas de Melo Ave, 21. Boa Vista, Vila Velha, Espirito Santo, 29102‑920, Brazil.
Inflammopharmacology. 2021 Oct;29(5):1443-1458. doi: 10.1007/s10787-021-00862-3. Epub 2021 Sep 21.
Alternanthera brasiliana (L.) Kuntze is recognized for its healing properties; however, its therapeutic effects remain unclear. Therefore, our study aimed to elucidate the wound healing activities of A. brasiliana using in vitro and in vivo assays. In vitro assays were used to evaluate the antibacterial, anti-inflammatory, and antioxidant effects of A. brasiliana extract. For the in vivo study, two dorsal excisions were established in Wistar rats using a punch (1.5 cm in diameter), which were topically treated daily with 2% carbopol gel (Ctrl group) or 20% hydroalcoholic plant extract with 2% carbopol gel (A. brasiliana-Ab group). After the 2nd, 7th, 14th, and 21st days, inflammation, oxidative damage, antioxidants, angiogenesis, tissue formation, and re-epithelialization were evaluated. In vitro, Ab reduced nitric oxide, anion superoxide, and pro-inflammatory cytokine production. In vivo, Ab presented lower levels of inflammatory infiltrate, although increased levels of IL-1β and TGF-β1 were observed. The plant extract controlled oxidative damage by antioxidants, which favored angiogenesis, collagenesis, and wound re-epithelialization. Thus, the topical application of the hydroalcoholic extract of 20% A. brasiliana was distinguished by its important anti-inflammatory and antioxidant activities both in vivo and in vitro. The plant extract also stimulated angiogenesis and tissue formation, accelerating total re-epithelization, which is promising for wound healing.
巴西野牡丹（Alternanthera brasiliana）因其具有治疗特性而被认可；然而，其治疗效果尚不清楚。因此，我们的研究旨在使用体外和体内试验来阐明巴西野牡丹的伤口愈合活性。体外试验用于评估巴西野牡丹提取物的抗菌、抗炎和抗氧化作用。在体内研究中，使用打孔器（直径 1.5 厘米）在 Wistar 大鼠的两个背部建立了两个切除部位，每天用 2%卡波姆凝胶（Ctrl 组）或 20%水醇植物提取物加 2%卡波姆凝胶（巴西野牡丹-Ab 组）进行局部处理。在第 2、7、14 和 21 天，评估炎症、氧化损伤、抗氧化剂、血管生成、组织形成和再上皮化。在体外，Ab 降低了一氧化氮、阴离子超氧化物和促炎细胞因子的产生。在体内，Ab 表现出较低水平的炎症浸润，尽管观察到 IL-1β和 TGF-β1 的水平增加。植物提取物通过抗氧化剂控制氧化损伤，这有利于血管生成、胶原形成和伤口再上皮化。因此，20%巴西野牡丹水醇提取物的局部应用因其在体内和体外的重要抗炎和抗氧化活性而脱颖而出。该植物提取物还刺激血管生成和组织形成，加速总上皮再形成，这对伤口愈合有很大的希望。
