Department of Medical Morphological Research, Medical School, University of Udine, Udine, Italy.
Fertil Steril. 2011 Mar 1;95(3):928-35. doi: 10.1016/j.fertnstert.2010.08.027.
To compare the ultrastructural appearance of oocytes after vitrification and warming with two different devices.
Oocytes were examined by ultrastructural analysis after vitrification and warming with use of closed (CryoTip; Irvine Scientific, Santa Ana, CA) or open (Cryotop; Kitazato BioPharma Co., Ltd., Shizuoka, Japan) devices.
Pordenone Hospital IVF Unit and Medical Morphological Research Department, University of Udine.
PATIENT(S): Surplus oocytes from 10 patients (aged 31-39 years) undergoing assisted reproductive technologies at the Pathophysiology Unit of Human Reproduction and Sperm Bank between 2006 and 2008.
INTERVENTION(S): Oocytes with normal invertoscopic appearance underwent vitrification and warming with closed (CryoTip) or open (Cryotop) devices and were processed for transmission electron microscopy.
MAIN OUTCOME MEASURE(S): Cryodamage extent and cell alterations in oocytes after open or closed vitrification and warming procedures and their rehydration rate.
RESULT(S): A higher rate of complete oocyte rehydration and less-severe ultrastructural alterations were observed after vitrification and warming with the open Cryotop device.
CONCLUSION(S): These preliminary data suggest that oocyte ultrastructure is better preserved with an open rather than closed vitrification and warming protocol.
比较两种不同装置玻璃化冷冻和复温后卵母细胞的超微结构外观。
使用封闭（CryoTip；Irvine Scientific，Santa Ana，CA）或开放（Cryotop；Kitazato BioPharma Co.，Ltd.，Shizuoka，Japan）装置对玻璃化冷冻和复温后的卵母细胞进行超微结构分析。
Pordenone 医院 IVF 单位和乌迪内大学医学形态学研究系。
2006 年至 2008 年期间，在人类生殖病理生理学单位和精子库接受辅助生殖技术的 10 名（年龄 31-39 岁）患者的多余卵母细胞。
采用封闭（CryoTip）或开放（Cryotop）装置对具有正常倒置镜外观的卵母细胞进行玻璃化冷冻和复温，并进行透射电子显微镜处理。
开放或封闭玻璃化冷冻和复温后卵母细胞的冰晶损伤程度和细胞变化及其复水率。
与封闭的 CryoTip 装置相比，使用开放的 Cryotop 装置进行玻璃化冷冻和复温后，卵母细胞的完全复水率更高，超微结构改变更轻微。
这些初步数据表明，开放的玻璃化冷冻和复温方案比封闭的方案更能保存卵母细胞的超微结构。
