Gene Regulation Section, Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, 251 Bayview Blvd., Room 06C214, Baltimore, MD 21224, United States.
Semin Immunol. 2010 Dec;22(6):337-45. doi: 10.1016/j.smim.2010.08.003.
The immunoglobulin heavy chain (IgH) gene locus is activated at a precise stage of B lymphocyte development to undergo gene rearrangements that assemble the functional gene. In this review we summarize our current understanding of the chromatin state of the IgH as it appears just prior to the initiation of V(D)J recombination, and the implications of this structure for regulation of recombination. We also examine the role of the intron enhancer, Eμ, in establishing the pre-rearrangement chromatin structure. The emerging picture shows that the IgH locus consists of independently regulated domains, each of which requires multiple levels of epigenetic changes to reach the fully activated state.
免疫球蛋白重链 (IgH) 基因座在 B 淋巴细胞发育的精确阶段被激活，以进行基因重排，从而组装功能基因。在这篇综述中，我们总结了我们目前对 IgH 染色质状态的理解，即在 V(D)J 重组开始之前，以及这种结构对重组调控的影响。我们还研究了内含子增强子 Eμ在建立预重排染色质结构中的作用。新兴的图景表明，IgH 基因座由独立调控的结构域组成，每个结构域都需要多个层次的表观遗传变化才能达到完全激活状态。
