The Coca-Cola Company, Research and Technology, Atlanta, GA 30313, USA.
Exp Biol Med (Maywood). 2010 Oct;235(10):1185-93. doi: 10.1258/ebm.2010.010063. Epub 2010 Sep 23.
In vitro models have been invaluable in determining the mechanisms involved in adipocyte proliferation, differentiation, adipokine secretion and gene/protein expression. The cells presently available for research purposes all have unique advantages and disadvantages that one should be aware of when selecting cells. Established cell lines, such as 3T3-L1 cells, are easier and less costly to use than freshly isolated cells, even though freshly isolated cells allow for various comparisons such as the in vitro evaluation of different in vivo conditions that may not be possible using cell lines. Moreover, stem cells, transdifferentiated cells or dedifferentiated cells are relatively new cell models being evaluated for the study of adipocyte regulation and physiology. The focus of this brief review is to highlight similarities and differences in adipocyte models to aid in appropriate model selection and data interpretation for successful advancement of our understanding of adipocyte biology.
在确定脂肪细胞增殖、分化、脂肪因子分泌和基因/蛋白表达所涉及的机制方面，体外模型一直具有不可估量的价值。目前可用于研究目的的细胞都有独特的优缺点，在选择细胞时应注意这些优缺点。与新鲜分离的细胞相比，3T3-L1 等已建立的细胞系使用起来更容易且成本更低，尽管新鲜分离的细胞可以进行各种比较，例如体外评估不同的体内条件，而这在使用细胞系时可能是不可能的。此外，干细胞、转分化细胞或去分化细胞是相对较新的细胞模型，正在被评估用于研究脂肪细胞的调节和生理学。本综述的重点是强调脂肪细胞模型的相似性和差异性，以帮助进行适当的模型选择和数据解释，从而成功地推进我们对脂肪细胞生物学的理解。
