Montaldo Laura, Bendezu Meza Llerson, De Marzi Mauricio, Guerra Liliana Noemi
Departamento de Ciencias Basicas, Universidad Nacional de Lujan, Av Constitucion y Ruta 5, Lujan 6700, Buenos Aires, Argentina.
CONICET-INEDES, Grupo de Investigaciones Basicas y Aplicadas en Inmunologıa y Bioactivos (GIBAIB), Av Constitucion y Ruta 5, Lujan 6700, Buenos Aires, Argentina.
Int J Mol Sci. 2025 May 2;26(9):4326. doi: 10.3390/ijms26094326.
Obesity is a metabolic condition of epidemic scale. Previously, we showed that antioxidant extracts from had antioxidant and anti-adipogenic effects in mature adipocytes (AD). Here, we evaluated an aqueous extract from (Boldo) in AD and studied its effect on reactive oxygen species (ROS) and lipid production. We analyzed the antioxidant activity (AA) of the Boldo extract using the DPPH technique and polyphenol (Pph) content via Folin's reagent. In AD, we evaluated ROS production, catalase (CAT) activity, intracellular triglyceride (Tg) and cholesterol (Chol) contents, nitric oxide (NO) production via Griess reagent, and the levels of glycerol (Gly) and TNF-α released in the culture medium. We showed that the Boldo extract has high AA. In vitro, Boldo treatment decreased ROS intracellular production and CAT activity. In addition, the Boldo extract was effective in reducing Tg and Chol levels and NO production. We did not identify significant differences in Gly released or TNF-α secreted. We suggest that the Boldo extract has antioxidant and anti-adipogenic effects, but we did not observe lipolytic effects. Boldo did not modify inflammatory markers.
肥胖是一种具有流行规模的代谢状况。此前，我们表明来自[具体来源未提及]的抗氧化提取物在成熟脂肪细胞（AD）中具有抗氧化和抗脂肪生成作用。在此，我们评估了来自[具体来源未提及]（波尔多叶）的水提取物在脂肪细胞中的作用，并研究了其对活性氧（ROS）和脂质生成的影响。我们使用DPPH技术分析了波尔多叶提取物的抗氧化活性（AA），并通过福林试剂分析了多酚（Pph）含量。在脂肪细胞中，我们评估了ROS生成、过氧化氢酶（CAT）活性、细胞内甘油三酯（Tg）和胆固醇（Chol）含量、通过格里斯试剂检测的一氧化氮（NO）生成，以及培养基中释放的甘油（Gly）和肿瘤坏死因子-α（TNF-α）水平。我们发现波尔多叶提取物具有较高的抗氧化活性。在体外，波尔多叶处理降低了细胞内ROS生成和CAT活性。此外，波尔多叶提取物在降低Tg和Chol水平以及NO生成方面有效。我们未发现释放的Gly或分泌的TNF-α有显著差异。我们认为波尔多叶提取物具有抗氧化和抗脂肪生成作用，但未观察到脂解作用。波尔多叶未改变炎症标志物。
