University of Geneva, Switzerland.
Brief Funct Genomics. 2010 Dec;9(5-6):362-73. doi: 10.1093/bfgp/elq023. Epub 2010 Sep 23.
Specific binding of transcription factors (TFs) determines in a large part the connectivity of gene regulatory networks as well as the quantitative level of gene expression. A multiplicity of both experimental and computational methods is currently used to discover and characterize the underlying TF-DNA interactions. Experimental methods can be further subdivided into in vitro- and in vivo-based approaches, each accenting different aspects of TF-binding events. In this review we summarize the flexibility and performance of a selection of both types of experimental methods. In conclusion, we argue that a serial combination of methods with different throughput and data type constitutes an optimal experimental strategy.
转录因子 (TFs) 的特异性结合在很大程度上决定了基因调控网络的连接性以及基因表达的定量水平。目前，有多种实验和计算方法可用于发现和描述潜在的 TF-DNA 相互作用。实验方法可进一步细分为基于体外和体内的方法，每种方法都强调 TF 结合事件的不同方面。在这篇综述中，我们总结了这两种类型的实验方法的灵活性和性能。总之，我们认为具有不同通量和数据类型的方法的串联组合是一种最佳的实验策略。
