Sztalryd C, Faust I M
Vassar College, Poughkeepsie, NY 12601.
Int J Obes. 1990;14 Suppl 3:165-75.
Variation in growth across an animal's fat depots derives, at less in part, from inter-depot differences in pre-adipocytes. However, because of technical difficulties that have impeded the study of pre-adipocytes in primary culture, these differences are not well defined. Adipose tissue stromal cell fractions that contain pre-adipocytes grow readily in culture but invariably include cells plated at various stages of maturity. Consequently, crucial events in relatively immature cells are often obscured by activities of cells that are more mature. Therefore, one aim of the present study was to diminish the impact of cells plated near maturity by maximizing the density of immature cells. This was accomplished by the plating of stromal-vascular (SV) cell fractions at low density. Since immature pre-adipocytes proliferate much more rapidly than do mature pre-adipocytes, they become predominant. This approach made it possible for a variety of differences to be seen between cells from retroperitoneal (RP) and epididymal (Epi) tissues: Epi, but not RP, cells were seen to assemble in multi-layer clumps; multiplication was found to remain constant through at least one subculture in RP cells, but not in Epi cells; and, in a variety of ways, RP cells showed more differentiation than Epi cells. The reasons for these differences remain to be determined, but just the fact that they exist suggests that important dissimilarities among pre-adipocytes (including those responsible for nonuniform growth of fat depots) can be revealed and studied in primary cell culture.
动物脂肪库之间生长的差异至少部分源于前脂肪细胞的库间差异。然而，由于技术上的困难阻碍了对原代培养前脂肪细胞的研究，这些差异尚未得到很好的界定。含有前脂肪细胞的脂肪组织基质细胞组分在培养中易于生长，但总是包含处于不同成熟阶段接种的细胞。因此，相对未成熟细胞中的关键事件常常被更成熟细胞的活动所掩盖。因此，本研究的一个目的是通过最大化未成熟细胞的密度来减少接近成熟时接种的细胞的影响。这是通过低密度接种基质血管（SV）细胞组分来实现的。由于未成熟的前脂肪细胞比成熟的前脂肪细胞增殖快得多，它们变得占主导地位。这种方法使得能够观察到来自腹膜后（RP）和附睾（Epi）组织的细胞之间的多种差异：Epi细胞（而非RP细胞）会聚集形成多层团块；发现RP细胞在至少一次传代培养中增殖保持恒定，而Epi细胞则不然；并且，在多种方面，RP细胞比Epi细胞表现出更多的分化。这些差异的原因尚待确定，但它们的存在这一事实表明，在前脂肪细胞之间（包括那些导致脂肪库生长不均一的细胞）的重要差异可以在原代细胞培养中被揭示和研究。
