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的过表达增加了红花中喹诺查耳酮的积累。 （注：原文中“Increases”前缺少具体基因或蛋白等相关内容，翻译只能做到此程度，需结合上下文补充完整准确意思）

Overexpression of Increases Accumulation of Quinochalcone in Safflower.

作者信息

Guo Dandan, Xue Yingru, Li Dongqiao, He Beixuan, Jia Xinlei, Dong Xin, Guo Meili

机构信息

Department of Pharmacognosy, College of Pharmacy, Second Military Medical UniversityShanghai, China.

Testing and Analysis Center, College of Pharmacy, Second Military Medical UniversityShanghai, China.

出版信息

Front Plant Sci. 2017 Aug 15;8:1409. doi: 10.3389/fpls.2017.01409. eCollection 2017.

DOI:10.3389/fpls.2017.01409
PMID:28861095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5559696/
Abstract

, the dried petal of safflower ( L.) has been widely used in traditional Chinese medicine to treat cardiovascular and cerebrovascular diseases, in which quinochalcone glucosides such as hydrosafflower yellow A (HSYA), carthamin are uniquely present and have been identified as active compounds. In the present study, through sequencing of a safflower floret cDNA library and subsequent microarray analysis, we found 23 unigenes (5 , 1 , 5 , 6 , 2 , 2 , 2 ) involved in flavonoid pathway, of which 4 were up-regulated differentially during quinochalcone glucosides accumulation with the floret developing stage. The up-regulated genes were verified by PCR methods. Considering chalcone synthase are entry enzyme in flavonoid biosynthesis, was focused on target gene to verify its function furtherly. Bioinformation analysis showed that shared 86.94% conserved residues with from other plants. Subcellular localization showed that was localized in cytoplasm in onion epidermal cells. The transgenic safflower plant with overexpression by -mediated pollen-tube pathway method was firstly generated. The results present that expression of , , increased and expression of and decreased in the transgenic plant floret. Meanwhile, the accumulation of quinochalcone glucosides increased by ∼20-30% and accumulation of quercetin-3-β-D-glucoside and quercetin decreased by 48 and 63% in the transgenic plant floret. These results suggested that played an important role in quinochalcone glucosides biosynthesis rather than flavonol biosynthesis. These results also demonstrated that the pollen-tube pathway method was an efficient method for gene transformation in safflower. Our study will provide a deep understanding of potential synthetic genes involved in quinochalcone biosynthetic pathway.

摘要

红花（Carthamus tinctorius L.）干燥花瓣在传统中药中被广泛用于治疗心脑血管疾病，其中含有独特的喹诺查耳酮苷，如水红花黄色素A（HSYA）、红花苷，这些已被鉴定为活性化合物。在本研究中，通过对红花小花cDNA文库进行测序及随后的微阵列分析，我们发现23个单基因（5个、1个、5个、6个、2个、2个、2个）参与类黄酮途径，其中4个在喹诺查耳酮苷积累过程中随小花发育阶段差异上调。上调基因通过PCR方法进行了验证。鉴于查耳酮合酶是类黄酮生物合成的起始酶，将其作为目标基因进一步验证其功能。生物信息分析表明，其与其他植物的查耳酮合酶有86.94%的保守残基。亚细胞定位表明其定位于洋葱表皮细胞的细胞质中。首次通过农杆菌介导的花粉管通道法获得了过表达该基因的转基因红花植株。结果表明，转基因植株小花中该基因、其他相关基因的表达增加，另外两个基因的表达下降。同时，转基因植株小花中喹诺查耳酮苷的积累增加约~20 - 30%，槲皮素-3-β-D-葡萄糖苷和槲皮素的积累分别下降48%和63%。这些结果表明该基因在喹诺查耳酮苷生物合成中起重要作用，而非黄酮醇生物合成。这些结果还表明花粉管通道法是红花基因转化的有效方法。我们的研究将为深入了解喹诺查耳酮生物合成途径中潜在的合成基因提供帮助。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/7e20ce3560dc/fpls-08-01409-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/977ac6712c8f/fpls-08-01409-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/b442882e7d38/fpls-08-01409-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/452cf325d6e1/fpls-08-01409-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/e3d9ab42e3ba/fpls-08-01409-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/b493c571486e/fpls-08-01409-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/16eea44d62cd/fpls-08-01409-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/9feed3e950de/fpls-08-01409-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/a63323d5b5ce/fpls-08-01409-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/abf8c19b423f/fpls-08-01409-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/3ab5c2035678/fpls-08-01409-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b7/5559696/7e20ce3560dc/fpls-08-01409-g012.jpg

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