Systems Biology Department, Harvard Medical School, Boston, MA, USA.
Wiley Interdiscip Rev Syst Biol Med. 2011 May-Jun;3(3):347-56. doi: 10.1002/wsbm.120. Epub 2010 Sep 23.
Protein microarray technology has emerged as a powerful tool for comparing binding interactions, expression level, substrate specificities, and posttranslational modifications (PTMs) of different proteins in a parallel and high-throughput manner. The ability to immobilize proteins to a solid surface and register the specific address of each protein has bridged major limitations for investigating the proteome in biological samples, namely, the wide dynamic range of protein concentrations and the perturbation of the physical and chemical properties of proteins by their modification. Recent advances introduced the use of functional mammalian cell extracts to assay PTMs under different cellular conditions. This assay offers a new approach for performing large-scale complex biochemical analysis of protein modifications. Here, we review studies of PTM profiling using protein microarrays and discuss the limitations and potential applications of the system. We believe that the information generated from such proteomic studies may be of significant value in our elucidation of the molecular mechanisms that govern human physiology.
蛋白质微阵列技术已经成为一种强大的工具，用于以平行和高通量的方式比较不同蛋白质的结合相互作用、表达水平、底物特异性和翻译后修饰（PTMs）。将蛋白质固定在固体表面上并记录每个蛋白质的特定地址的能力，克服了研究生物样品中蛋白质组的主要限制，即蛋白质浓度的宽动态范围以及蛋白质的物理和化学性质因修饰而受到干扰。最近的进展引入了使用功能性哺乳动物细胞提取物在不同细胞条件下测定 PTM 的方法。该测定为进行大规模复杂的蛋白质修饰生化分析提供了一种新方法。在这里，我们综述了使用蛋白质微阵列进行 PTM 分析的研究，并讨论了该系统的局限性和潜在应用。我们相信，从这种蛋白质组学研究中获得的信息可能对我们阐明控制人体生理学的分子机制具有重要价值。
