Mustafa Shakhawan Abdulrahman, Hoheisel Jörg D, Alhamdani Mohamed Saiel Saeed
Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
Mol Biosyst. 2011 Jun;7(6):1795-801. doi: 10.1039/c1mb05071k. Epub 2011 Apr 20.
Following the advances in human genome sequencing, attention has shifted in part toward the elucidation of the encoded biological functions. Since proteins are the driving forces behind very many biological activities, large-scale examinations of their expression variations, their functional roles and regulation have moved to the central stage. A significant fraction of the human proteome consists of secreted proteins. Exploring this set of molecules offers unique opportunities for understanding molecular interactions between cells and fosters biomarker discovery that could advance the detection and monitoring of diseases. Antibody microarrays are among the relatively new proteomic methodologies that may advance the field significantly because of their relative simplicity, robust performance and high sensitivity down to single-molecule detection. In addition, several aspects such as variations in amount, structure and activity can be assayed at a time. Antibody microarrays are therefore likely to improve the analytical capabilities in proteomics and consequently permit the production of even more informative and reliable data. This review looks at recent applications of this novel platform technology in secretome analysis and reflects on the future.
随着人类基因组测序技术的进步，人们的注意力部分转向了对编码的生物学功能的阐释。由于蛋白质是众多生物活动背后的驱动力，对其表达变化、功能作用和调控的大规模研究已成为核心内容。人类蛋白质组中有很大一部分是分泌蛋白。探索这组分子为理解细胞间的分子相互作用提供了独特的机会，并促进了生物标志物的发现，这可能会推动疾病的检测和监测。抗体微阵列是相对较新的蛋白质组学方法之一，由于其相对简单、性能稳健且灵敏度高至单分子检测，可能会极大地推动该领域的发展。此外，诸如数量、结构和活性等几个方面可以同时进行检测。因此，抗体微阵列可能会提高蛋白质组学的分析能力，从而产生更丰富、更可靠的数据。本综述探讨了这种新型平台技术在分泌蛋白质组分析中的最新应用，并对未来进行了思考。
