Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
Clin Exp Rheumatol. 2010 Jul-Aug;28(4 Suppl 60):S16-9. Epub 2010 Sep 23.
Behçet's disease (BD) is a helper T cell-mediated autoimmune disease characterised by recurrent orogenital ulcers, uveitis and skin lesions. The helper T cells are divided into Th1, Th2 and Th17 cells according to the pattern of cytokine secretion. Th1 and Th17 cells can contribute to the development of the disease with their respective proinflammatory cytokines, IFN-γ and IL-17. In this study, we investigated the relative role of Th17, Th1 and Th2 cells in BD.
Peripheral blood mononuclear cells were isolated from 30 patients with BD, for whom the detailed clinical manifestations and medication history were investigated and recorded. Surface markers and intracellular levels of IL-17, IFN-γ and IL-4 in isolated CD4+ T cells were measured using flow-cytometry from these patients and from two control groups, 34 rheumatoid arthritis patients and 24 healthy blood donors to analyse the relationship of Th1, Th17 and Th2 cells in BD.
The ratio of Th17/Th1 cells was significantly increased in BD compared to healthy controls (0.16±0.09 vs. 0.10±0.04, p=0.012), while there was no difference in the ratios of Th1/Th2 or Th17/Th2 cells. Th17/Th1 ratio was elevated in BD patients with uveitis or folliculitis compared to those without it (0.21±0.10 vs. 0.13±0.06, p=0.045 for uveitis; 0.18±0.10 vs. 0.12±0.05, p=0.036 for folliculitis).
Our results confirm that TH17 cells are instrumental in Behçet's uveitis and folliculitis. Furthermore, our findings suggest that the role of Th17 cells should be interpreted in the context of their ratio to Th1 cells.
贝切特病（BD）是一种辅助 T 细胞介导的自身免疫性疾病，其特征为复发性口腔或生殖器溃疡、葡萄膜炎和皮肤损伤。辅助 T 细胞根据细胞因子分泌模式分为 Th1、Th2 和 Th17 细胞。Th1 和 Th17 细胞可通过各自的促炎细胞因子 IFN-γ 和 IL-17 促进疾病的发展。本研究旨在探讨 Th17、Th1 和 Th2 细胞在 BD 中的相对作用。
从 30 例 BD 患者中分离外周血单个核细胞，详细记录患者的临床表现和用药史。通过流式细胞术检测分离的 CD4+T 细胞中 IL-17、IFN-γ 和 IL-4 的表面标志物和细胞内水平，分析 BD 患者与 34 例类风湿关节炎患者和 24 例健康献血者之间 Th1、Th17 和 Th2 细胞的关系。
与健康对照组相比，BD 患者 Th17/Th1 细胞比例明显升高（0.16±0.09 比 0.10±0.04，p=0.012），而 Th1/Th2 或 Th17/Th2 细胞比例无差异。葡萄膜炎或毛囊炎患者的 Th17/Th1 比值高于无该两种疾病的患者（0.21±0.10 比 0.13±0.06，p=0.045 对于葡萄膜炎；0.18±0.10 比 0.12±0.05，p=0.036 对于毛囊炎）。
我们的结果证实 Th17 细胞在 BD 的葡萄膜炎和毛囊炎中起作用。此外，我们的发现表明，应该根据 Th17 细胞与 Th1 细胞的比值来解释 Th17 细胞的作用。
