Valera Marcia Carneiro, da Rosa Jucely Aparecida, Maekawa Lilian Eiko, de Oliveira Luciane Dias, Carvalho Cláudio Antonio Talge, Koga-Ito Cristiane Yumi, Jorge Antonio Olavo Cardoso
Department of Restorative Dentistry, School of Dentistry of São José dos Campos, São Paulo State University, São José dos Campos, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Oct;110(4):e70-4. doi: 10.1016/j.tripleo.2010.01.029.
This study evaluated the action of propolis and intracanal medications against Escherichia coli and endotoxin. Forty-eight dental roots were contaminated with E. coli. The root canals were instrumented with propolis and divided into groups according to the type of intracanal medication: Ca(OH)(2), polymyxin B, or Ca(OH)(2) + 2% chlorhexidine gel. In the control group, saline solution was used without application of intracanal medication. Counts of colony-forming units were carried out and the endotoxin was quantified by the chromogenic Limulus amobocyte lysate assay. The results were evaluated by analysis of variance and the Dunn test (5%). Root canal irrigation with propolis was effective to completely eliminate E. coli and reduce the amount of endotoxins. All intracanal medications contributed to the significant decrease in endotoxins. Only intracanal medications may reduce the amount of endotoxins in the root canals. The greatest efficacy was observed for medications containing Ca(OH)(2).
本研究评估了蜂胶和根管内用药对大肠杆菌及内毒素的作用。48颗牙根被大肠杆菌污染。用蜂胶对根管进行预备，并根据根管内用药类型分为几组：氢氧化钙、多粘菌素B或氢氧化钙+2%氯己定凝胶。对照组使用生理盐水，不应用根管内用药。进行菌落形成单位计数，并通过显色鲎试剂法对内毒素进行定量。结果通过方差分析和邓恩检验（5%）进行评估。用蜂胶进行根管冲洗可有效完全清除大肠杆菌并减少内毒素量。所有根管内用药均有助于显著降低内毒素量。只有根管内用药可减少根管内的内毒素量。含氢氧化钙的药物观察到最大疗效。
