Restorative Dentistry Department, Univ. Estadual Paulista, São José dos Campos, São Paulo, SP, Brazil.
J Appl Oral Sci. 2013 Mar-Apr;21(2):118-23. doi: 10.1590/1678-7757201302135.
The aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis inoculated in root canals.
Seventy-two human tooth roots were contaminated with C. albicans and E. faecalis for 21 days. The groups were divided according to the auxiliary chemical substance into: G1) 2.5% sodium hypochlorite (NaOCl), G2) 2% chlorhexidine gel (CHX), G3) castor oil, G4) glycolic Aloe vera extract, G5) glycolic ginger extract, and G6) sterile saline (control). The samples of the root canal were collected at different intervals: confirmation collection, at 21 days after contamination; 1st collection, after instrumentation; and 2nd collection, seven days after instrumentation. Microbiological samples were grown in culture medium and incubated at 37°C for 48 hours.
The results were submitted to the Kruskal-Wallis and Dunn (5%) statistical tests. NaOCl and CHX completely eliminated the microorganisms of the root canals. Castor oil and ginger significantly reduced the number of CFU of the tested bacteria. Reduction of CFU/mL at the 1st and 2nd collections for groups G1, G2, G3 and G4 was greater in comparison to groups G5 and G6.
It was concluded that 2.5% sodium hypochlorite and 2% chlorhexidine gel were more effective in eliminating C. albicans and E. faecalis, followed by the castor oil and glycolic ginger extract. The Aloe vera extract showed no antimicrobial activity.
本研究旨在评估辅助化学物质和天然提取物对接种于根管内的白色念珠菌和粪肠球菌的抗菌活性。
将 72 个人类牙根污染白色念珠菌和粪肠球菌，持续 21 天。根据辅助化学物质将组分为：G1）2.5%次氯酸钠（NaOCl）、G2）2%洗必泰凝胶（CHX）、G3）蓖麻油、G4） 乙醇酸芦荟提取物、G5）乙醇酸生姜提取物和 G6）无菌生理盐水（对照组）。在不同时间点采集根管样本：确认采集，污染后 21 天；1 次采集，器械处理后；2 次采集，器械处理后 7 天。微生物样本在培养皿中培养，并在 37°C 下孵育 48 小时。
结果采用 Kruskal-Wallis 和 Dunn（5%）统计检验。NaOCl 和 CHX 完全消除了根管内的微生物。蓖麻油和生姜显著降低了测试细菌的 CFU 数量。与 G5 和 G6 组相比，G1、G2、G3 和 G4 组在 1 次和 2 次采集时 CFU/mL 的减少更为明显。
研究结论认为，2.5%次氯酸钠和 2%洗必泰凝胶在消除白色念珠菌和粪肠球菌方面更为有效，其次是蓖麻油和乙醇酸生姜提取物。芦荟提取物没有抗菌活性。
