College of Nanoscale Science and Engineering, University at Albany, 257 Fuller Road, Albany, NY, USA.
J Biotechnol. 2010 Nov;150(3):312-4. doi: 10.1016/j.jbiotec.2010.09.946. Epub 2010 Sep 30.
In this work we propose a novel method of immobilizing nucleic acids for field effect or high electron mobility transistor-based biosensors. The naturally occurring 5' terminal phosphate group on nucleic acids was used to coordinate with semiconductor and metal oxide surfaces. We demonstrate that DNA can be directly immobilized onto ZrO(2), AlGaN, GaN, and HfO(2) while retaining its ability to hybridize to target sequences with high specificity. By directly immobilizing the probe molecule to the sensor surface, as opposed to conventional crosslinking strategies, the number of steps in device fabrication is reduced. Furthermore, hybridization to target strands occurs closer to the sensor surface, which has the potential to increase device sensitivity by reducing the impact of the Debye screening length.
在这项工作中，我们提出了一种将核酸固定在基于场效应或高电子迁移率晶体管的生物传感器上的新方法。核酸的天然 5'端磷酸基团用于与半导体和金属氧化物表面配位。我们证明，DNA 可以直接固定在 ZrO(2)、AlGaN、GaN 和 HfO(2)上，同时保持其与靶序列高特异性杂交的能力。通过将探针分子直接固定在传感器表面，而不是传统的交联策略，可以减少器件制造的步骤数。此外，由于杂交发生在离传感器表面更近的地方，这有可能通过减小德拜屏蔽长度的影响来提高器件的灵敏度。
