McGowan Institute for Regenerative Medicine, Department of Bioengineering, University of Pittsburgh, Bridgeside Point Building II, 450 Technology Dr. Suite 300, Pittsburgh, PA 15219, USA.
Biomaterials. 2011 Jan;32(1):128-36. doi: 10.1016/j.biomaterials.2010.09.006. Epub 2010 Sep 25.
Biologic scaffold materials composed of mammalian extracellular matrix (ECM) are commonly used for the repair and reconstruction of injured tissues. An important, but unexplored variable of biologic scaffolds is the age of the animal from which the ECM is prepared. The objective of the present study was to compare the structural, mechanical, and compositional properties of small intestinal submucosa (SIS)-ECM harvested from pigs that differed only in age. Degradation product bioactivity of these ECM materials was also examined. Results showed that there are distinct differences in each of these variables among the various age source ECM scaffolds. The strength and growth factors content of ECM from 3-week-old animals is less than that of ECM harvested from 12, 26 or >52-week-old animals. The elastic modulus of SIS-ECM for 3 week and >52-week-old source was less than that of the 12 and 26 week source. Degradation products from all age source ECMs were chemotactic for perivascular stem cells, with the 12 week source the most potent, while the oldest source caused the greatest increase in proliferation. In summary, distinct differences exist in the mechanical, structural, and biologic properties of SIS-ECM harvested from different aged animals.
由哺乳动物细胞外基质（ECM）组成的生物支架材料通常用于修复和重建受损组织。生物支架的一个重要但尚未探索的变量是 ECM 制备动物的年龄。本研究的目的是比较仅在年龄上有所不同的猪小肠黏膜下层（SIS）-ECM 的结构、力学和组成特性。还检查了这些 ECM 材料的降解产物生物活性。结果表明，在这些不同来源的 ECM 支架中，这些变量中的每一个都存在明显差异。来自 3 周龄动物的 ECM 的强度和生长因子含量小于来自 12、26 或>52 周龄动物的 ECM。3 周龄和>52 周龄来源的 SIS-ECM 的弹性模量小于 12 周龄和 26 周龄来源的弹性模量。所有年龄来源的 ECM 的降解产物对血管周围干细胞均具有趋化性，其中 12 周龄来源的趋化性最强，而最古老的来源则导致增殖增加最多。总之，从不同年龄的动物中提取的 SIS-ECM 在机械、结构和生物学特性方面存在明显差异。
