σ 因子（orf21）在棒状链霉菌 NRRL3585 中克拉维酸生产中的作用。
Role of σ-factor (orf21) in clavulanic acid production in Streptomyces clavuligerus NRRL3585.
机构信息
Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University, # 100, Asansi, Chungnam 336-708, Republic of Korea.
出版信息
Microbiol Res. 2011 Jul 20;166(5):369-79. doi: 10.1016/j.micres.2010.07.005. Epub 2010 Sep 25.
A putative sigma factor gene, orf21, was disrupted or overexpressed in the wild-type clavulanic acid (CA) producer Streptomyces clavuligerus NRRL3585 and characterized. An orf21 mutant (Streptomyces clavuligerus HN14) of S. clavuligerus was obtained by insertional inactivation via double-crossover. Although there was little reduction of sporulation in the mutant, the growth pattern was similar between mutant and wild-type. The production was reduced by 10-15% in S. clavuligerus HN14 compared to that in wild-type. Overexpression of orf21 in wild-type cells caused hyperproduction of spores on solid medium and increased clavulanic acid production by 1.43-fold. The overexpression of orf21 in wild-type S. clavuligerus stimulated the expression of the early clavulanic acid genes, ceas2 and cas2, and the regulatory gene, ccaR, as demonstrated by RT-PCR. The elevation of the ceas2, cas2 and ccaR transcripts was consistent with the enhanced production of clavulanic acid.
一个假定的 sigma 因子基因或 f21 在野生型克拉维酸（CA）产生菌棒状链霉菌 NRRL3585 中被破坏或过表达，并进行了表征。通过双交换插入失活获得了棒状链霉菌 orf21 突变体（棒状链霉菌 HN14）。尽管突变体中的孢子形成减少很少，但突变体和野生型之间的生长模式相似。与野生型相比，在 S. clavuligerus HN14 中的产量减少了 10-15%。orf21 在野生型细胞中的过表达导致固体培养基上孢子的过度产生，并使克拉维酸的产量增加了 1.43 倍。orf21 在野生型 S. clavuligerus 中的过表达通过 RT-PCR 证明了早期克拉维酸基因 ceas2 和 cas2 以及调节基因 ccaR 的表达。ceas2、cas2 和 ccaR 转录物的升高与克拉维酸产量的提高一致。
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