环境因素调节σ因子在ATCC27064中克拉维酸生产中的作用。
Environmental Factors Modulate the Role of Sigma Factor in Clavulanic Acid Production in ATCC27064.
作者信息
Patiño Luisa F, Aguirre-Hoyos Vanessa, Pinilla Laura I, Toro León F, Ríos-Estepa Rigoberto
机构信息
Grupo de Bioprocesos, Departamento de Ingeniería Química, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín 050010, Colombia.
出版信息
Bioengineering (Basel). 2022 Feb 16;9(2):78. doi: 10.3390/bioengineering9020078.
Sigma factors and sigma factor-related mechanisms control antibiotic production in . In this contribution, the gene was overexpressed in the wild-type strain of ATCC2764, yielding /pIORF21, to further evaluate its regulatory effect on clavulanic acid (CA) biosynthesis under different culture medium conditions. The overexpression, regulated under the constitutive promoter , led to 2.6-fold increase in CA production in GSPG medium, and a 1.8-fold decrease using ISP medium. As for GYM and MYM media, /pIORF21 strain showed higher aerial mycelium production compared to control. Glycerol uptake rate profile was affected by overexpression. Furthermore, in GSPG, /pIORF21 slightly increased the expression of and genes, whilst, in ISP, the gene expression was drastically reduced, which is consistent with a decreased CA production, observed in this medium. These findings suggest the protein encoded by the gene plays a role in the regulation of CA biosynthesis as a response to the nutritional composition of the medium.
西格玛因子及与西格玛因子相关的机制控制着……中的抗生素生产。在本研究中，……基因在……ATCC2764野生型菌株中过表达，产生……/pIORF21，以进一步评估其在不同培养基条件下对克拉维酸（CA）生物合成的调控作用。在组成型启动子……调控下的……过表达，导致在GSPG培养基中CA产量增加2.6倍，而在ISP培养基中则降低1.8倍。至于GYM和MYM培养基，……/pIORF21菌株与对照相比显示出更高的气生菌丝产量。甘油摄取速率曲线受……过表达的影响。此外，在GSPG中，……/pIORF21略微增加了……和……基因的表达，而在ISP中，……基因表达大幅降低，这与在该培养基中观察到的CA产量下降一致。这些发现表明，……基因编码的蛋白质在作为对培养基营养成分响应的CA生物合成调控中发挥作用。
Zotero 插件