Pinilla Laura, Toro León F, Laing Emma, Alzate Juan Fernando, Ríos-Estepa Rigoberto
Grupo de Bioprocesos, Universidad de Antioquia, Calle, Medellín 70 No. 52-21, Colombia.
Department of Microbial Sciences, School of Biosciences and Medicine, University of Surrey, Guildford GU2 7XH, UK.
Antibiotics (Basel). 2019 Jul 19;8(3):96. doi: 10.3390/antibiotics8030096.
Clavulanic acid (CA), a β-lactamase inhibitor, is industrially produced by the fermentation of . The efficiency of CA production is associated with media composition, culture conditions and physiological and genetic strain characteristics. However, the molecular pathways that govern CA regulation in remain unknown. Here we used RNA-seq to perform a comparative transcriptome analysis of ATCC 27064 wild-type strain grown in both a favorable soybean-based medium and in limited media conditions to further contribute to the understanding of metabolism and its regulation. A total of 350 genes were found to be differentially expressed between conditions; 245 genes were up-regulated in favorable conditions compared to unfavorable. The up-regulated expression of many regulatory and biosynthetic CA genes was positively associated with the favorable complex media condition along with pleiotropic regulators, including proteases and some genes whose biological function have not been previously reported. Knowledge from differences between transcriptomes from complex/defined media represents an advance in the understanding of regulatory paths involved in metabolic response, enabling the rational design of future experiments.
克拉维酸（CA）是一种β-内酰胺酶抑制剂，通过发酵工业生产。CA的生产效率与培养基组成、培养条件以及菌株的生理和遗传特性有关。然而，控制CA在[具体微生物名称未给出]中调控的分子途径仍不清楚。在这里，我们使用RNA测序对[具体微生物名称未给出]ATCC 27064野生型菌株在有利的大豆基培养基和有限培养基条件下生长进行了比较转录组分析，以进一步有助于理解[具体微生物名称未给出]的代谢及其调控。共发现350个基因在不同条件下差异表达；与不利条件相比，245个基因在有利条件下上调。许多调控和生物合成CA基因的上调表达与有利的复合培养基条件以及多效性调节因子呈正相关，包括蛋白酶和一些以前未报道过生物学功能的基因。来自复合/限定培养基转录组差异的知识代表了对[具体微生物名称未给出]代谢反应中涉及的调控途径理解的进步，有助于未来实验的合理设计。
