Mahapatra S, Banerjee D
Vidyasagar University, Department of Microbiology, Midnapore 721102, West Bengal, India.
Acta Microbiol Immunol Hung. 2010 Sep;57(3):215-23. doi: 10.1556/AMicr.57.2010.3.6.
Endophytic fungi of three tissues (petiole, bark and leaf) of Alstonia scholaris were assessed. A total number of 1,152 endophytic fungi were isolated from 1,002 different plant segments of seven different localities of Paschim Medinipur, West Bengal, India. The isolated fungi belong to nineteen genera, including four unidentified fungi and yeast. Colletotrichum sp. (20.39%) and Sordaria sp. (29.68%) were most commonly isolated from this plant. Hyalopus sp., Fusarium sp. and Curvularia sp. were also isolated. The colonization frequency of endophytic fungi is much higher in leaves (44.66%) in comparison to petioles (32.16%) and barks (23.17%). The study provided evidence for tissue specificity of endophytic fungi. The endophytic fungal species diversity was higher in plant segments collected from Gopegarh and Khoirullahchak, while diversity was the lowest in Rice mill area. Screenings of antimicrobial activity of these isolated endophytic fungi were done. Eight endophytic fungi showed antimicrobial activity. Among them Curvularia sp., Aspergillus sp. and one unidentified fungus showed maximum activity against test pathogens.
对印度西孟加拉邦帕斯奇姆梅迪尼布尔七个不同地区的鸡骨常山三种组织（叶柄、树皮和叶片）的内生真菌进行了评估。从1002个不同的植物片段中总共分离出1152株内生真菌，这些植物片段来自印度西孟加拉邦帕斯奇姆梅迪尼布尔七个不同地区。分离出的真菌属于19个属，包括4株未鉴定的真菌和酵母菌。炭疽菌属（20.39%）和粪壳菌属（29.68%）是从该植物中最常分离出的真菌。还分离出了透明孢属、镰刀菌属和弯孢属真菌。内生真菌在叶片中的定殖频率（44.66%）远高于叶柄（32.16%）和树皮（23.17%）。该研究为内生真菌的组织特异性提供了证据。从戈佩加尔和科伊鲁拉查克采集的植物片段中内生真菌物种多样性较高，而在碾米厂地区多样性最低。对这些分离出的内生真菌进行了抗菌活性筛选。8株内生真菌表现出抗菌活性。其中弯孢属、曲霉属和1株未鉴定的真菌对测试病原体表现出最大活性。
