Lashari Punhal, Laghari Muhammad Younis, Xu Peng, Zhao Zixia, Jiang Li, Narejo Naeem Tariq, Deng Yulin, Sun Xiaowen, Zhang Yan
a School of Life Sciences, Beijing Institute of Technology , Beijing , China .
b Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences , Beijing , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):387-8. doi: 10.3109/19401736.2014.895998. Epub 2014 Mar 11.
Complete mitochondrial genome of catfish, Sperata seenghala, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and red listed species. The complete mitogenome was 16,588 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 31.28% A, 27.80% C, 15.31% G, and 25.57% T. The complete mitochondrial genome of catfish, Sperata seenghala provides the fundamental tools for genetic breeding and conservation studies.
通过长片段PCR（宝生物LA Taq，中国大连）分离出鲶鱼（Sperata seenghala）的完整线粒体基因组；并采用桑格法进行测序以获得完整的线粒体基因组，该物种被列为极度濒危和红色名录物种。完整的线粒体基因组长度为16,588 bp，包含13个典型的脊椎动物蛋白质编码基因、2个rRNA基因和22个tRNA基因。全基因组碱基组成估计为A占31.28%、C占27.80%、G占15.31%、T占25.57%。鲶鱼（Sperata seenghala）的完整线粒体基因组为遗传育种和保护研究提供了基础工具。
