Skvortsova V I, Tvorogova T V, Dubina A I, Burenchev D V, Gubskiĭ L V, Stakhovskaia L V, Povarova O V
Zh Nevrol Psikhiatr Im S S Korsakova. 2009;109(12 Suppl 2):62-6.
The aim of this study was to examine Taftsin derivates--macrophage inhibitory factor (MIF, Thr-Lys-Pro) and heptapeptide selank (Thr-Lys-Pro-Arg-Pro-Gly-Pro) in the model of experimental intracerebral hemorrhage in rats. The double autologous blood injection in the basal nucleus was used as a model of intracerebral hemorrhage. Animals ware randomly divided into three groups--the control group (n = 5) was treated with saline, the second group (n = 5) was injected with MIF in dose 150 mkg/ kg/day, the third group (n = 5) received Selank in dose 300 mkg/kg/day. Intraperitoneal injection of peptides was used. Body weight assessment, neurological examination and brain MRI were performed in 24, 72 hours and 10 days after the hematoma formation. The effect of neuropeptides on the functional restoration in animals, in the absence of the effect on hematoma volume and perifocal edema, was found. The significant reduction of perifocal edema and hematoma volume was observed in the 10th day after the hematoma formation in all experimental groups (p < 0.05). Only the control group of animals showed the significant (p < 0.05) weight loss in the 3rd day after the operation. The rate of neurological deficit was different: the significant improvement assessed with Menzes and limb placing test scales was seen only in the groups treated with neuropeptides in the 10th day.
本研究旨在大鼠实验性脑出血模型中检测塔夫辛衍生物——巨噬细胞抑制因子（MIF，苏氨酸-赖氨酸-脯氨酸）和七肽selank（苏氨酸-赖氨酸-脯氨酸-精氨酸-脯氨酸-甘氨酸-脯氨酸）。将基底核内双自体血注射用作脑出血模型。动物被随机分为三组——对照组（n = 5）用生理盐水治疗，第二组（n = 5）以150μg/kg/天的剂量注射MIF，第三组（n = 5）以300μg/kg/天的剂量接受Selank。采用肽的腹腔注射。在血肿形成后24小时、72小时和10天进行体重评估、神经学检查和脑部MRI。发现神经肽对动物功能恢复有影响，而对血肿体积和灶周水肿无影响。在血肿形成后第10天，所有实验组均观察到灶周水肿和血肿体积显著减小（p < 0.05）。只有动物对照组在术后第3天出现显著（p < 0.05）体重减轻。神经功能缺损率不同：仅在第10天用神经肽治疗的组中，用门泽斯和肢体放置测试量表评估有显著改善。
