The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia.
The Department of Biomedical and Pharmaceutical Technology, Faculty of Biotechnology and Organic Synthesis, Lomonosov Moscow University of Fine Chemical Technology, 86 Vernadskogo Av, Moscow 119571, Russia.
Mol Immunol. 2014 Mar;58(1):50-5. doi: 10.1016/j.molimm.2013.11.002. Epub 2013 Nov 27.
Previous studies have shown that synthetic tuftsin analogue Selank and its fragments cause a number of alterations in the expression of certain genes involved in inflammation in mouse spleen. In this work we studied the effect of Selank and its short fragment Gly-Pro on the temporary dynamics of C3, Casp1, Il2rg, and Xcr1 genes expression in mouse spleen after single intraperitoneal injection (100 μg/kg) of peptides using real-time PCR method. We found a significant 3-fold decrease in the C3 mRNA level just 30 min after Selank injection and similar alteration this gene mRNA level after Gly-Pro administration. A wave-like alteration in the Casp1 mRNA level was observed after Selank injection. We found a significant alteration in the mRNA level of the Il2rg gene at early time points after Selank and Gly-Pro administration and an almost equal reduction in the Xcr1 mRNA level 90 min after the administration of Selank and its fragment. Our results showed that, Selank and its short fragment Gly-Pro influence the expression of genes that mediate different types of immune responses, thereby maintaining the balance of the immune system. It should be noted that in most cases, there was a coincidence in the expression profiles of the studied genes after Selank and Gly-Pro administration. This might indicate an active contribution of the dipeptide to the final effect of Selank.
先前的研究表明，合成的 tuftsin 类似物 Selank 及其片段会引起小鼠脾脏中参与炎症的某些基因表达的一系列改变。在这项工作中，我们使用实时 PCR 方法研究了 Selank 及其短片段 Gly-Pro 对单次腹腔注射（100μg/kg）肽后小鼠脾脏中 C3、Casp1、Il2rg 和 Xcr1 基因表达的暂时动态的影响。我们发现，在注射 Selank 后仅 30 分钟，C3 mRNA 水平显著下降了 3 倍，而在给予 Gly-Pro 后，该基因的 mRNA 水平也发生了类似的改变。在注射 Selank 后，Casp1 mRNA 水平呈现出波浪式的改变。我们发现，在 Selank 和 Gly-Pro 给药后的早期时间点，Il2rg 基因的 mRNA 水平发生了显著改变，而在 Selank 和其片段给药 90 分钟后，Xcr1 mRNA 水平几乎相等。我们的研究结果表明，Selank 和其短片段 Gly-Pro 影响介导不同类型免疫反应的基因的表达，从而维持免疫系统的平衡。需要注意的是，在大多数情况下，在给予 Selank 和 Gly-Pro 后，研究基因的表达谱是一致的。这可能表明二肽对 Selank 的最终效果有积极的贡献。
