Smith D J, King W F, Taubman M A
Forsyth Dental Center, Boston, MA.
Oral Microbiol Immunol. 1990 Apr;5(2):57-62. doi: 10.1111/j.1399-302x.1990.tb00228.x.
Salivary IgA antibodies to oral streptococci which colonize the infant oral cavity soon after birth were analyzed in 53 whole salivas of 17 infants who were between three and 27 weeks old. Antibody activities to Streptococcus mitis cells were detected by enzyme linked immunosorbent assay in 78% of the whole salivas by the twelfth week of age. This antibody activity was associated with polymeric IgA as determined after gel filtration of salivas on Superose 6, followed by ELISA. Western blot analyses were used to detect IgA antibodies to Streptococcus mitis and Streptococcus salivarius culture supernatants. Forty one, and 91% of saliva samples contained IgA antibody which reacted in Western blot analyses with S. salivarius and S. mitis culture supernatants, respectively. The youngest infant to show reactive IgA antibody with either oral streptococcal antigen preparation was five week old. Salivary IgA antibody to either bacterial culture supernatant was detected in Western blot only after the isolation of the respective streptococcal species from the oral cavity of these young infants. Some heterogeneity was observed among patterns developed with salivas from different infants. These results suggest that salivary IgA antibody responses may be induced by oral colonization (S. mitis, S. salivarius) by the end of the first month of life.
对17名3至27周龄婴儿的53份全唾液样本分析了针对出生后不久即定植于婴儿口腔的口腔链球菌的唾液IgA抗体。在12周龄时，通过酶联免疫吸附测定法在78%的全唾液样本中检测到了针对缓症链球菌细胞的抗体活性。经唾液在Superose 6上进行凝胶过滤后再进行ELISA测定，发现这种抗体活性与聚合IgA有关。采用蛋白质印迹分析检测针对缓症链球菌和唾液链球菌培养上清液的IgA抗体。分别有41%和91%的唾液样本中含有在蛋白质印迹分析中与唾液链球菌和缓症链球菌培养上清液发生反应的IgA抗体。显示出与任何一种口腔链球菌抗原制剂发生反应的IgA抗体的最年幼婴儿为5周龄。只有在从这些幼儿口腔中分离出相应的链球菌种类后，才能在蛋白质印迹分析中检测到针对任何一种细菌培养上清液的唾液IgA抗体。不同婴儿的唾液样本所形成的模式存在一些异质性。这些结果表明，在生命的第一个月末，唾液IgA抗体反应可能由口腔定植（缓症链球菌、唾液链球菌）所诱导。
