Widerstrom L, Bratthall D, Hamberg K
Department of Cariology, Faculty of Odontology, University of Lund, Sweden.
Oral Microbiol Immunol. 1992 Dec;7(6):326-31. doi: 10.1111/j.1399-302x.1992.tb00631.x.
Antigen extracts of 9 strains of Streptococcus mutans, Streptococcus sobrinus, Streptococcus salivarius and Streptococcus mitis were separated in acrylamide gels by electrophoresis. Strains of the same genotype of mutans streptococci were found to have virtually identical sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns but differed clearly from other genotypes of mutans or with the reference strains S. salivarius and S. mitis. Immunoglobulin A (IgA) antibody activity to antigens of S. mutans, S. sobrinus and S. mitis in the parotid, submandibular and whole saliva of 12 people was detected by Western blotting. IgA antibodies reacted with several of the antigens, and the reaction pattern was unique for each of the individuals. To certain antigens, all the salivas blotted bands if both clear and questionable bands were counted. Significantly more blotted bands were revealed with antigens from S. mutans than S. sobrinus. The results of the 3 different salivas of the same individual had a high degree of resemblance but in a few individuals, more blotted bands were obtained with parotid saliva to S. mutans and S. sobrinus antigens.
采用聚丙烯酰胺凝胶电泳法，对9株变形链球菌、远缘链球菌、唾液链球菌和缓症链球菌的抗原提取物进行分离。结果发现，变形链球菌相同基因型的菌株具有几乎相同的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱，但与变形链球菌的其他基因型或参考菌株唾液链球菌和缓症链球菌明显不同。采用蛋白质印迹法检测了12名受试者腮腺、颌下腺及全唾液中针对变形链球菌、远缘链球菌和缓症链球菌抗原的免疫球蛋白A（IgA）抗体活性。IgA抗体与多种抗原发生反应，且每个个体的反应模式都是独特的。对于某些抗原，如果将清晰条带和可疑条带都计算在内，所有唾液均出现印迹条带。变形链球菌抗原显示出的印迹条带明显多于远缘链球菌。同一受试者的3种不同唾液检测结果具有高度相似性，但少数受试者的腮腺唾液针对变形链球菌和远缘链球菌抗原产生了更多的印迹条带。
