University of Campinas, Campinas, São Paulo, Brazil.
J Med Food. 2010 Dec;13(6):1385-90. doi: 10.1089/jmf.2009.0256. Epub 2010 Sep 27.
Collagen hydrolysates (CHs) are mixtures of peptides obtained by partial hydrolysis of gelatins that are receiving scientific attention as potential oral supplements for the recovery of osteoarticular tissues. The effect of supplementing the diets with a CH was assessed in 48 ovariectomized rats by analyzing the compositional and biomechanical characteristics of the bone. Six groups of rats (three ovariectomized, one sham-operated, and two intact) were fed a standard diet, supplemented with either CH or gelatin (Control), at two levels: a dose equivalent to five times the amount suggested for humans (10 g/day) or another 10 times greater. After 8 weeks, the femora and vertebrae were excised, the blood was collected, and serum alkaline phosphatase and osteocalcin were determined. Bone weight, total protein, and biomechanical strength were also determined. The vertebrae of the ovariectomized group that received the higher dosage of CH withstood a load four times greater and exhibited higher levels of protein and osteocalcin content than those receiving either gelatin or no supplement. CH supplementation at the higher level in the ovariectomized rat had an unequivocal contribution in the conservation or preservation of vertebral mass, protein content, and mechanical strength not seen when gelatin was used as a supplement. Similar treatment of the intact rat with the CH, however, appeared to have the opposite effect.
胶原蛋白水解物（CHs）是通过明胶部分水解得到的肽混合物，由于其作为潜在的关节组织修复口服补充剂的作用正受到科学界的关注。本研究通过分析骨的组成和生物力学特性，评估了在 48 只去卵巢大鼠日粮中添加 CH 的效果。将 6 组大鼠（3 只去卵巢，1 只假手术，2 只完整）喂食标准饮食，并分别用 CH 或明胶（对照）补充，剂量分别相当于建议人类摄入量的 5 倍（10 g/天）和 10 倍。8 周后，取出股骨和椎骨，采集血液，测定血清碱性磷酸酶和骨钙素。还测定了骨重、总蛋白和生物力学强度。与接受明胶或不补充的大鼠相比，接受 CH 高剂量补充的去卵巢大鼠的椎骨能承受 4 倍的负荷，且具有更高的蛋白和骨钙素含量。在去卵巢大鼠中，CH 高剂量补充对维持或保留椎骨质量、蛋白含量和机械强度有明确作用，而用明胶作为补充剂则没有这种作用。然而，用 CH 对完整大鼠进行类似的处理，其效果似乎相反。
