Adeghate E, Donáth T
1st Department of Anatomy, Histology and Embryology, Semmelweis University Medical School, Budapest, Hungary.
Peptides. 1990 Nov-Dec;11(6):1087-92. doi: 10.1016/0196-9781(90)90135-r.
Neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) immunoreactive nerves were demonstrated in 21-day-old embryonic pancreatic tissue fragments transplanted into the anterior eye chamber of rats for 22, 45 and 109 days and in 60-day-old normal adult pancreas using immunohistochemical technique. In normal adult tissue, NPY-positive neurons lie close to the basal and lateral walls of the acinar cells. NPY-containing nerve fiber plexuses were found around blood vessels. VIP-immunopositive nerves were also discernible in the outer parts of the islets of Langerhans and on pancreatic ducts. In the transplants, it is not only the neural elements that survived but also the pancreatic ducts and the endocrine cells. VIP- and NPY-positive neurons were found in the stroma of the surviving pancreatic tissue. The distribution of these neural elements is similar to that of normal tissue in the surviving pancreatic ducts but different with regards to the acinar tissue. This study confirms that intrinsic nerves can survive and synthesize polypeptides even after 109 days of transplantation into the anterior eye chamber.
采用免疫组织化学技术，在移植到大鼠眼前房22天、45天和109天的21日龄胚胎胰腺组织碎片以及60日龄正常成年胰腺中，证实了神经肽Y（NPY）和血管活性肠多肽（VIP）免疫反应性神经的存在。在正常成年组织中，NPY阳性神经元靠近腺泡细胞的基底壁和侧壁。在血管周围发现了含NPY的神经纤维丛。在胰岛外部和胰管上也可辨别出VIP免疫阳性神经。在移植组织中，不仅存活的是神经成分，还有胰管和内分泌细胞。在存活的胰腺组织基质中发现了VIP和NPY阳性神经元。这些神经成分在存活的胰管中的分布与正常组织相似，但在腺泡组织方面有所不同。本研究证实，即使在前房移植109天后，内在神经仍可存活并合成多肽。
