Berger Z, Fehér E
Second Department of Internal Medicine, St Imre Hospital, Budapest, Hungary.
Int J Pancreatol. 1997 Apr;21(2):165-71. doi: 10.1007/BF02822388.
These results provide morphological evidence for an alcohol-induced selective intrapancreatic nerve degeneration. This affected mainly the nerve fibers that are inhibitory of the exocrine pancreas, and might represent the morphological background of hypersecretory state of the pancreas in chronic alcoholism.
Intrapancreatic intrinsic nerves were studied by immunohistochemistry and electron microscopy after 4 mo of alcohol consumption and compared with control mice.
A dense network of nerve fibers was observed in the normal mouse pancreas around the blood vessels and ending on the exocrine cells. The presence of VIP, NPY, PP, SP, and serotonin in these nerves was demonstrated by immunohistochemistry. Four months of alcohol consumption did not result in apparent morphological changes of the pancreas. However, the majority of periacinar nerve terminals showed degenerative changes. Synaptic vesicles were diminished in number in some other nerve processes, whereas the perivascular nerve fibers were relatively well preserved. A slight decrease was found in the intensity of VIP and SP immunoreactivity, and the PP fibers almost disappeared.
这些结果为酒精诱导的胰腺内神经选择性变性提供了形态学证据。这主要影响了对胰腺外分泌起抑制作用的神经纤维，可能代表了慢性酒精中毒时胰腺高分泌状态的形态学背景。
在小鼠饮酒4个月后，通过免疫组织化学和电子显微镜研究胰腺内固有神经，并与对照小鼠进行比较。
在正常小鼠胰腺中，观察到血管周围有密集的神经纤维网络，并终止于外分泌细胞。免疫组织化学证实这些神经中存在血管活性肠肽（VIP）、神经肽Y（NPY）、胰多肽（PP）、P物质（SP）和5-羟色胺。饮酒4个月并未导致胰腺出现明显的形态学变化。然而，大多数腺泡周围神经末梢呈现退行性改变。在其他一些神经突起中，突触小泡数量减少，而血管周围神经纤维相对保存完好。发现VIP和SP免疫反应强度略有降低，PP纤维几乎消失。
