Agency for Science, Research and Technology, Singapore.
Emerg Infect Dis. 2010 Oct;16(10):1633-6. doi: 10.3201/eid1610.100688.
An oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus evolved and emerged from zero to 52% of detectable virus within 48 hours of a patient's exposure to oseltamivir. Phylogenetic analysis and data gathered by pyrosequencing and cloning directly on clinical samples suggest that the mutant emerged de novo.
奥司他韦耐药的 2009 年甲型 H1N1 流感大流行病毒在患者接触奥司他韦后 48 小时内从零进化到可检测病毒的 52%。系统进化分析和通过焦磷酸测序和直接在临床样本上克隆获得的数据表明,该突变是从头出现的。