Servicio de Cirugía Cardiaca, Hospital Clínico Universitario, Santiago de Compostela, A Coruña, España.
Rev Esp Cardiol. 2010 Oct;63(10):1205-8. doi: 10.1016/s1885-5857(10)70236-x.
With a novel real-time multiplex polymerase chain reaction test, the LightCycler SeptiFast® test, 25 bacterial and fungal species can be identified directly in blood. The SeptiFast® test has been used for rapid etiologic diagnosis in infectious endocarditis using blood samples but has not been evaluated directly on cardiac vegetations in patients being treated for infectious endocarditis. We prospectively analyzed 15 samples of heart valve tissue with active infectious endocarditis using the SeptiFast® test and compared the test's sensitivity with that of blood culture, valve tissue culture, and the SeptiFast® test in blood. The sensitivity of the SeptiFast test in heart valve tissue was 100%. The test results confirmed the diagnosis obtained using blood culture in 13 cases and identified the pathogen in 2 cases where blood culture tested negative. The sensitivity of the SeptiFast® test in heart valve tissue was greater than that obtained with conventional culture of vegetations or with the SeptiFast test in blood.
采用新型实时多重聚合酶链反应检测技术——LightCycler SeptiFast®检测，可直接从血液中鉴定 25 种细菌和真菌种类。SeptiFast®检测已用于感染性心内膜炎的快速病因诊断，采用的是血样标本，但尚未直接在接受感染性心内膜炎治疗的患者的心脏赘生物上进行评估。我们前瞻性地分析了 15 例患有活动性感染性心内膜炎的心脏瓣膜组织样本，采用 SeptiFast®检测，并将该检测的敏感性与血培养、瓣膜组织培养以及血液中的 SeptiFast®检测进行比较。SeptiFast 检测在心脏瓣膜组织中的敏感性为 100%。检测结果在 13 例中证实了血培养获得的诊断，并在 2 例血培养阴性的病例中确定了病原体。与传统的瓣膜组织培养或血液中的 SeptiFast 检测相比，SeptiFast®检测在心脏瓣膜组织中的敏感性更高。
