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Rapid Microbiological Diagnostics from Explanted Heart Valves by a Multiplex PCR Assay.

作者信息

Bast Antje, Dohmen Pascal M, Podbielski Andreas, Warnke Philipp

机构信息

Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany.

Department of Cardiac Surgery, Heart Center Rostock, University Medicine Rostock, Rostock, Germany.

出版信息

J Clin Microbiol. 2019 Jan 30;57(2). doi: 10.1128/JCM.01575-18. Print 2019 Feb.

DOI:10.1128/JCM.01575-18
PMID:30429258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6355538/
Abstract
摘要

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Augmented recovery of microorganisms from swabs by homogenization: a novel standardizable high-throughput approach.通过匀浆增强拭子中微生物的回收率：一种新型标准化高通量方法。
Diagn Microbiol Infect Dis. 2016 Jan;84(1):16-18. doi: 10.1016/j.diagmicrobio.2015.10.004. Epub 2015 Oct 9.
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Infective Endocarditis in Adults: Diagnosis, Antimicrobial Therapy, and Management of Complications: A Scientific Statement for Healthcare Professionals From the American Heart Association.成人感染性心内膜炎：诊断、抗菌治疗和并发症处理：美国心脏协会医疗保健专业人员科学声明。
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2015 ESC Guidelines for the management of infective endocarditis: The Task Force for the Management of Infective Endocarditis of the European Society of Cardiology (ESC). Endorsed by: European Association for Cardio-Thoracic Surgery (EACTS), the European Association of Nuclear Medicine (EANM).2015年欧洲心脏病学会（ESC）感染性心内膜炎管理指南：欧洲心脏病学会（ESC）感染性心内膜炎管理工作组。认可机构：欧洲心胸外科学会（EACTS）、欧洲核医学协会（EANM）。
Eur Heart J. 2015 Nov 21;36(44):3075-3128. doi: 10.1093/eurheartj/ehv319. Epub 2015 Aug 29.
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Improved microbiological diagnostic due to utilization of a high-throughput homogenizer for routine tissue processing.由于在常规组织处理中使用了高通量均质器，微生物诊断得到了改善。
Diagn Microbiol Infect Dis. 2015 Jul;82(3):189-93. doi: 10.1016/j.diagmicrobio.2015.03.018. Epub 2015 Apr 6.
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A commercially available multiplex real-time PCR for detection of pathogens in cardiac valves from patients with infective endocarditis.一种用于检测感染性心内膜炎患者心脏瓣膜中病原体的市售多重实时聚合酶链反应。
Diagn Microbiol Infect Dis. 2014 May;79(1):98-101. doi: 10.1016/j.diagmicrobio.2013.12.011. Epub 2013 Dec 21.
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Evaluation of a multiplex real-time PCR assay for detecting pathogens in cardiac valve tissue in patients with endocarditis.评价一种用于检测心内膜炎患者心瓣膜组织中病原体的多重实时 PCR 检测方法。
Rev Esp Cardiol. 2010 Oct;63(10):1205-8. doi: 10.1016/s1885-5857(10)70236-x.
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The changing face of infective endocarditis.感染性心内膜炎的面貌变迁
Heart. 2006 Jul;92(7):879-85. doi: 10.1136/hrt.2005.067256. Epub 2005 Oct 10.
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Infective endocarditis due to unusual or fastidious microorganisms.由不常见或苛求菌引起的感染性心内膜炎。
Mayo Clin Proc. 1997 Jun;72(6):532-42. doi: 10.4065/72.6.532.
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Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies.用于定量分子微生物生态学研究的通用小亚基rRNA杂交探针的特性分析
Appl Environ Microbiol. 1996 Dec;62(12):4504-13. doi: 10.1128/aem.62.12.4504-4513.1996.
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16S ribosomal DNA amplification for phylogenetic study.用于系统发育研究的16S核糖体DNA扩增。
J Bacteriol. 1991 Jan;173(2):697-703. doi: 10.1128/jb.173.2.697-703.1991.