Afshari Arash, Schrenzel Jacques, Ieven Margareta, Harbarth Stephan
Crit Care. 2012 May 29;16(3):222. doi: 10.1186/cc11202.
Among critically ill patients, the diagnosis of bloodstream infection poses a major challenge. Current standard bacterial identification based on blood culture platforms is intrinsically time-consuming and slow. The continuous evolvement of molecular techniques has the potential of providing a faster, more sensitive and direct identification of causative pathogens without prior need for cultivation. This may ultimately impact clinical decision-making and antimicrobial treatment. This review summarises the currently available technologies, their strengths and limitations and the obstacles that have to be overcome in order to develop a satisfactory bedside point-of-care diagnostic tool for detection of bloodstream infection.
在重症患者中，血流感染的诊断是一项重大挑战。目前基于血培养平台的标准细菌鉴定本质上耗时且缓慢。分子技术的不断发展有可能在无需预先培养的情况下，更快、更灵敏且直接地鉴定致病病原体。这最终可能会影响临床决策和抗菌治疗。本综述总结了目前可用的技术、它们的优势与局限性，以及为开发出一种用于检测血流感染的令人满意的床边即时诊断工具而必须克服的障碍。
