Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China.
Analyst. 2012 Nov 21;137(22):5382-6. doi: 10.1039/c2an35962f. Epub 2012 Oct 5.
In this study, we demonstrate a more sensitive colorimetric assay for the detection of melamine based on the strong attraction between exocyclic amine groups of melamine and surface-bound AuCl(4)(-)/AuCl(2)(-) ions on bare gold nanoparticles prepared by the borohydride reduction method. This method is rather simple without further modification of gold nanoparticles and complicated pretreatment of samples. The detection limit of the method is 2.0 × 10(-7) g L(-1) for melamine in infant formula, which is much lower (about 1000-fold) than that of using citrate-capped GNPs as a colorimetric probe. The proposed scheme could be a good alternative means for on-site and real-time screening of melamine adulterant in infant formula without costly instruments.
在这项研究中，我们展示了一种更灵敏的基于三聚氰胺外环己胺基团与通过硼氢化钠还原法制备的金纳米粒子表面结合的 AuCl(4)(-) / AuCl(2)(-)离子之间强烈吸引力的比色法检测三聚氰胺的方法。该方法非常简单，不需要对金纳米粒子进行进一步修饰，也不需要对样品进行复杂的预处理。该方法对婴儿配方奶粉中三聚氰胺的检测限为 2.0 × 10(-7) g L(-1)，比使用柠檬酸封端的 GNPs 作为比色探针低得多（约 1000 倍）。该方案无需昂贵的仪器即可作为现场和实时筛查婴儿配方奶粉中三聚氰胺掺杂物的替代手段。
