Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing, China.
J Zhejiang Univ Sci B. 2012 Jul;13(7):525-32. doi: 10.1631/jzus.B1100389.
In recent years, there were two reported outbreaks of food borne illness associated with melamine. The presence of melamine and its related compounds in milk, feed, and other foods has resulted in the need for reliable methods for the detection and accurate quantification of this class of contaminants. The sample pretreatment for melamine in a complex matrix usually involves a liquid extraction by a polar solvent, followed by a further clean-up with solid phase extraction. Analyses of melamine and related compounds are commonly carried out by liquid or gas chromatographic methods conjugated with mass spectrometry. Other innovative screening methods, which use antibodies, molecularly imprinted polymers, capillary electrophoresis, and gold nanoparticles, are also used to develop assays and biosensors to melamine. However, many of these methods have been hindered by matrix effects, the solubility of melamine-cyanuric acid complex, and background contamination. This article reviews recent developments for detecting melamine and discusses future directions.
近年来，有两起因三聚氰胺引起的食源性疾病爆发的报道。三聚氰胺及其相关化合物在牛奶、饲料和其他食品中的存在，导致需要可靠的方法来检测和准确量化这一类污染物。在复杂基质中检测三聚氰胺的样品预处理通常涉及用极性溶剂进行液液萃取，然后用固相萃取进一步净化。三聚氰胺及其相关化合物的分析通常通过与质谱联用的液相或气相色谱方法进行。其他创新的筛选方法，如使用抗体、分子印迹聚合物、毛细管电泳和金纳米粒子，也被用于开发三聚氰胺的测定和生物传感器。然而，这些方法中的许多都受到基质效应、三聚氰胺-三聚氰酸络合物的溶解度和背景污染的阻碍。本文综述了近年来检测三聚氰胺的最新进展，并讨论了未来的发展方向。
