Phillips Melissa M, Bedner Mary, Reitz Manuela, Burdette Carolyn Q, Nelson Michael A, Yen James H, Sander Lane C, Rimmer Catherine A
Chemical Sciences Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899-8392, USA.
Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 18, 72076, Tübingen, Germany.
Anal Bioanal Chem. 2017 Feb;409(4):949-960. doi: 10.1007/s00216-016-9997-7. Epub 2016 Nov 10.
Two independent analytical approaches, based on liquid chromatography with absorbance detection and liquid chromatography with mass spectrometric detection, have been developed for determination of isoflavones in soy materials. These two methods yield comparable results for a variety of soy-based foods and dietary supplements. Four Standard Reference Materials (SRMs) have been produced by the National Institute of Standards and Technology to assist the food and dietary supplement community in method validation and have been assigned values for isoflavone content using both methods. These SRMs include SRM 3234 Soy Flour, SRM 3236 Soy Protein Isolate, SRM 3237 Soy Protein Concentrate, and SRM 3238 Soy-Containing Solid Oral Dosage Form. A fifth material, SRM 3235 Soy Milk, was evaluated using the methods and found to be inhomogeneous for isoflavones and unsuitable for value assignment. Graphical Abstract Separation of six isoflavone aglycones and glycosides found in Standard Reference Material (SRM) 3236 Soy Protein Isolate.
已开发出两种基于液相色谱吸光度检测和液相色谱质谱检测的独立分析方法，用于测定大豆制品中的异黄酮。对于多种大豆基食品和膳食补充剂，这两种方法得出的结果相当。美国国家标准与技术研究院制备了四种标准参考物质（SRM），以协助食品和膳食补充剂领域进行方法验证，并已使用这两种方法为异黄酮含量赋值。这些SRM包括SRM 3234大豆粉、SRM 3236大豆分离蛋白、SRM 3237大豆浓缩蛋白和SRM 3238含大豆的固体口服剂型。使用这些方法对第五种物质SRM 3235豆浆进行了评估，发现其异黄酮不均匀，不适用于赋值。图摘要标准参考物质（SRM）3236大豆分离蛋白中六种异黄酮苷元和糖苷的分离。
