Turinsky Andrei L, Turner Brian, Borja Rosanne C, Gleeson James A, Heath Michael, Pu Shuye, Switzer Thomas, Dong Dong, Gong Yunchen, On Tuan, Xiong Xuejian, Emili Andrew, Greenblatt Jack, Parkinson John, Zhang Zhaolei, Wodak Shoshana J
Hospital for Sick Children, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada.
Nucleic Acids Res. 2011 Jan;39(Database issue):D889-94. doi: 10.1093/nar/gkq857. Epub 2010 Sep 28.
Chromatin modification (CM) is a set of epigenetic processes that govern many aspects of DNA replication, transcription and repair. CM is carried out by groups of physically interacting proteins, and their disruption has been linked to a number of complex human diseases. CM remains largely unexplored, however, especially in higher eukaryotes such as human. Here we present the DAnCER resource, which integrates information on genes with CM function from five model organisms, including human. Currently integrated are gene functional annotations, Pfam domain architecture, protein interaction networks and associated human diseases. Additional supporting evidence includes orthology relationships across organisms, membership in protein complexes, and information on protein 3D structure. These data are available for 962 experimentally confirmed and manually curated CM genes and for over 5000 genes with predicted CM function on the basis of orthology and domain composition. DAnCER allows visual explorations of the integrated data and flexible query capabilities using a variety of data filters. In particular, disease information and functional annotations are mapped onto the protein interaction networks, enabling the user to formulate new hypotheses on the function and disease associations of a given gene based on those of its interaction partners. DAnCER is freely available at http://wodaklab.org/dancer/.
染色质修饰（CM）是一组表观遗传过程，它调控DNA复制、转录和修复的多个方面。CM由相互作用的蛋白质组执行，其破坏与许多复杂的人类疾病有关。然而，CM在很大程度上仍未被探索，特别是在人类等高等真核生物中。在这里，我们展示了DAnCER资源，它整合了来自包括人类在内的五种模式生物中具有CM功能的基因信息。目前整合的内容包括基因功能注释、Pfam结构域架构、蛋白质相互作用网络以及相关的人类疾病。其他支持证据包括跨生物体的直系同源关系、蛋白质复合物成员身份以及蛋白质三维结构信息。这些数据可用于962个经实验证实和人工整理的CM基因，以及基于直系同源关系和结构域组成预测具有CM功能的5000多个基因。DAnCER允许使用各种数据过滤器对整合数据进行可视化探索并具备灵活的查询功能。特别是，疾病信息和功能注释被映射到蛋白质相互作用网络上，使用户能够根据给定基因的相互作用伙伴的功能和疾病关联，对该基因的功能和疾病关联提出新的假设。DAnCER可在http://wodaklab.org/dancer/免费获取。
