Australian Herbicide Resistance Initiative, School of Plant Biology, UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.
Heredity (Edinb). 2011 May;106(5):817-24. doi: 10.1038/hdy.2010.124. Epub 2010 Sep 29.
The dynamics of herbicide resistance evolution in plants are influenced by many factors, especially the biochemical and genetic basis of resistance. Herbicide resistance can be endowed by enhanced rates of herbicide metabolism because of the activity of cytochrome P450 enzymes, although in weedy plants the genetic control of cytochrome P450-endowed herbicide resistance is poorly understood. In this study we have examined the genetic control of P450 metabolism-based herbicide resistance in a well-characterized Lolium rigidum biotype. The phenotypic resistance segregation in herbicide resistant and susceptible parents, F1, F2 and backcross (BC) families was analyzed as plant survival following treatment with the chemically unrelated herbicides diclofop-methyl or chlorsulfuron. Dominance and nuclear gene inheritance was observed in F1 families when treated at the recommended field doses of both herbicides. The segregation values of P450 herbicide resistance phenotypic traits observed in F2 and BC families was consistent with resistance endowed by two additive genes in most cases. In obligate out-crossing species such as L. rigidum, herbicide selection can easily result in accumulation of resistance genes within individuals.
植物中除草剂抗性进化的动力学受到许多因素的影响，特别是抗性的生化和遗传基础。由于细胞色素 P450 酶的活性，除草剂代谢率的增强可以赋予除草剂抗性，尽管杂草植物中细胞色素 P450 赋予的除草剂抗性的遗传控制还了解甚少。在这项研究中，我们研究了在一个特征明确的 Lolium rigidum 生物型中基于 P450 代谢的除草剂抗性的遗传控制。在用化学上无关的除草剂 diclofop-methyl 或 chlorosulfuron 处理后，对具有抗药性和敏感性亲本、F1、F2 和回交（BC）群体中的表型抗性分离进行了分析。当以两种除草剂的推荐田间剂量处理 F1 群体时，观察到显性和核基因遗传。在大多数情况下，F2 和 BC 群体中观察到的 P450 除草剂抗性表型性状的分离值与由两个加性基因赋予的抗性一致。在像 Lolium rigidum 这样的强制性异交物种中，除草剂选择很容易导致个体内抗性基因的积累。
