Ludwig-Maximilians-University Munich, Department of Chemistry, Physical Chemistry I, Butenandtstr. 11, Gerhard-Ertl-Gebäude, 81377 Munich, Germany.
Small. 2010 Nov 5;6(21):2427-35. doi: 10.1002/smll.201000762.
The increasing exposure of humans to nanoscaled particles requires well-defined systems that enable the investigation of the toxicity of nanoparticles on the cellular level. To facilitate this, surface-labeled silica nanoparticles, nanoparticles with a labeled core and a silica shell, and a labeled nanoparticle network-all designed for live-cell imaging-are synthesized. The nanoparticles are functionalized with perylene derivatives. For this purpose, two different perylene species containing one or two reactive silica functionalities are prepared. The nanoparticles are studied by transmission electron microscopy, widefield and confocal fluorescence microscopy, as well as by fluorescence spectroscopy in combination with fluorescence anisotropy, in order to characterize the size and morphology of the nanoparticles and to prove the success and homogeneity of the labeling. Using spinning-disc confocal measurements, silica nanoparticles are demonstrated to be taken up by HeLa cells, and they are clearly detectable inside the cytoplasm of the cells.
人类接触纳米级颗粒的情况日益增多，因此需要有明确界定的系统来研究纳米颗粒对细胞层面的毒性。为促进这一目标的实现，合成了表面标记的二氧化硅纳米颗粒、标记核和二氧化硅壳的纳米颗粒以及标记纳米颗粒网络，这些都是为活细胞成像而设计的。纳米颗粒用苝衍生物进行功能化。为此，制备了两种不同的含有一个或两个反应性硅功能基团的苝类物质。通过透射电子显微镜、宽场和共焦荧光显微镜以及荧光光谱学结合荧光各向异性对纳米颗粒进行研究，以表征纳米颗粒的大小和形态，并证明标记的成功和均匀性。通过旋转盘共聚焦测量，证明了二氧化硅纳米颗粒被 HeLa 细胞摄取，并且在细胞细胞质内清晰可检测。
