College of Life Sciences, Nankai University, Tianjin 300071, China.
Phytother Res. 2010 Oct;24(10):1451-6. doi: 10.1002/ptr.3120.
Memory performance, brain excitatory amino acid and acetylcholinesterase activity of chronically aluminum (Al) exposed mice in response to soy isoflavones (SI) treatment was investigated in the study. Forty eight mice were allotted randomly into a control group, an Al exposed group (100 mg/kg Al) and an Al exposed group treated with SI (100 mg/kg Al + 60 mg/kg SI) for 60 days. Chronic Al exposure significantly impaired long memory performance in mice as assessed using a passive avoidance task test (χ(2) analysis, p < 0.05). Interestingly, SI treatment markedly improved the memory performance score in the Al exposed mice. This improvement was associated with a total reversal of Al-induced increases in acetylcholinesterase activity in the cerebral cortex and hippocampus of mice. The Al exposure also led to significant decreases in brain levels of aspartic and glutamic acids, two excitatory amino acid neurotransmitters; whereas SI treatment partially reversed the decreased aspartic and glutamic acid contents in the hippocampus. The results suggest that SI can improve long memory performance in the Al exposed mice, possibly by modulating the metabolism of brain acetylcholine and amino acid neurotransmitters.
本研究探讨了长期暴露于铝（Al）的小鼠在接受大豆异黄酮（SI）治疗后的记忆表现、大脑兴奋性氨基酸和乙酰胆碱酯酶活性。48 只小鼠被随机分配到对照组、Al 暴露组（100mg/kg Al）和 Al 暴露组加 SI 治疗组（100mg/kg Al+60mg/kg SI），共处理 60 天。通过被动回避任务测试（卡方分析，p＜0.05）发现，慢性 Al 暴露显著损害了小鼠的长时记忆表现。有趣的是，SI 治疗显著提高了 Al 暴露小鼠的记忆表现评分。这种改善与大脑皮层和海马中乙酰胆碱酯酶活性的增加有关，Al 暴露导致大脑中两种兴奋性氨基酸神经递质天冬氨酸和谷氨酸的水平显著降低；而 SI 治疗部分逆转了海马中降低的天冬氨酸和谷氨酸含量。结果表明，SI 可改善 Al 暴露小鼠的长时记忆表现，可能通过调节大脑乙酰胆碱和氨基酸神经递质的代谢。
