Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Armed-Forces Taichung General Hospital, Taichung, Taiwan. Republic of China.
Yonsei Med J. 2010 Nov;51(6):924-31. doi: 10.3349/ymj.2010.51.6.924.
To distinguish lupus flare-up from infection in systemic lupus erythematosus (SLE), we analyze the expression of circulating CD27(high) plasma cells in SLE patients with and without infection, in comparison to non-SLE patients with infection.
The percentage of circulating CD27(high) plasma cells was measured by flow cytometry in the following four groups: 36 SLE patients without infection, 23 SLE patients with infection, eight non-SLE patients with infection, and 26 healthy controls.
The frequency of CD27(high) plasma cells had a correlation with the SLE disease activity index (SLEDAI) (r = 0.866, p < 0.05), level of anti-dsDNA (r = 0.886, p < 0.05), C3 (r = - 0.392, p < 0.05), and C4 (r = - 0.337, p < 0.05) in SLE patients without infection, but there was no correlation with disease activity in SLE patients with infection. Among three groups in particular-SLE without infection, SLE with infection, and non-SLE with infection- the percentages of CD27(high) plasma cells were elevated. The percentage of CD27(high) plasma cells was higher in SLE patients with infection, when compared to SLE patients without infection.
The percentage of CD27(high) plasma cells is a biomarker for disease activity of SLE without infection, under correlation with SLEDAI, anti-dsDNA, and C3 and C4 level. However, when the SLE patients have an infection, the percentage of CD27(high) plasma cells is not an adequate biomarker for the survey of disease activity. The percentage of CD27(high) plasma cells may serve as a potential parameter to distinguish a lupus flare-up from infection.
为了区分系统性红斑狼疮（SLE）患者的狼疮发作与感染，我们分析了伴有和不伴有感染的 SLE 患者以及伴有感染的非 SLE 患者循环中 CD27（高）浆细胞的表达。
通过流式细胞术测量以下四组患者循环中 CD27（高）浆细胞的百分比：36 例无感染的 SLE 患者、23 例感染的 SLE 患者、8 例感染的非 SLE 患者和 26 例健康对照者。
无感染的 SLE 患者中，CD27（高）浆细胞的频率与 SLE 疾病活动指数（SLEDAI）呈正相关（r = 0.866，p < 0.05），与抗 dsDNA 呈正相关（r = 0.886，p < 0.05），与 C3 呈负相关（r = - 0.392，p < 0.05），与 C4 呈负相关（r = - 0.337，p < 0.05）。但在感染的 SLE 患者中，与疾病活动无相关性。在无感染的 SLE、感染的 SLE 和感染的非 SLE 三组患者中，CD27（高）浆细胞的百分比均升高。与无感染的 SLE 患者相比，感染的 SLE 患者的 CD27（高）浆细胞比例更高。
无感染的 SLE 患者中，CD27（高）浆细胞的百分比与 SLEDAI、抗 dsDNA 以及 C3 和 C4 水平相关，是疾病活动的生物标志物。但是，当 SLE 患者发生感染时，CD27（高）浆细胞的百分比不能作为疾病活动的充分标志物。CD27（高）浆细胞的百分比可作为区分狼疮发作与感染的潜在参数。
