Font J, Pallares L, Martorell J, Martinez E, Gaya A, Vives J, Ingelmo M
Unitat de Malalties Autoimmunes Sistemiques, Hospital Clínic, Barcelona, Spain.
Clin Immunol Immunopathol. 1996 Dec;81(3):239-43. doi: 10.1006/clin.1996.0184.
The CD27 belongs to the NGF-R, a family of type I transmembrane receptors present in most T cells. The soluble form of CD27 can be found in body fluids, and elevation of serum levels is observed in T cell activation. Preliminary studies indicate that measurement of sCD27 levels might be of use in the evaluation of immune responses in vivo. Systemic lupus erythematosus (SLE) is a disease of unknown etiology. T cells from SLE patients have impaired responsiveness to various kinds of stimuli. However, the precise localization of the defect in the activation of SLE remains unclear. In this study we have analyzed the sCD27 serum levels in a SLE population and in a healthy group control. Seventy patients with SLE were prospectively studied. As controls, 20 healthy volunteer blood donors, matched by sex and age, were studied. We developed an enzyme-linked immunosorbent assay (ELISA) to measure sCD27. The mean level (+/-SD) of sCD27 was higher in the SLE patients (48.29 +/- 23.86 u) than that in the control group (36.13 +/- 7.48 u) (P = 0.02). In addition, patients with active SLE revealed higher serum concentration of sCD27 (58.20 +/- 31.06 u) than that of patients in remission (42.77 +/- 16.71 u) (P < 0.01). We found a positive correlation among sCD27 and sCD25 serum levels in SLE patients (r = 0.30, P = 0.01). No significant relation was found for other clinical symptoms or immunological parameters. In conclusion, sCD27 serum levels were increased in SLE patients and this increase was associated with the activity of the disease. Our data are consistent with the hypothesis that the CD27 antigen may constitute a marker of disease activity.
CD27属于NGF-R，是一类存在于大多数T细胞中的I型跨膜受体家族。CD27的可溶性形式可在体液中发现，并且在T细胞活化时血清水平会升高。初步研究表明，测量sCD27水平可能有助于评估体内免疫反应。系统性红斑狼疮（SLE）是一种病因不明的疾病。SLE患者的T细胞对各种刺激的反应性受损。然而，SLE激活缺陷的确切定位仍不清楚。在本研究中，我们分析了SLE患者群体和健康对照组的血清sCD27水平。对70例SLE患者进行了前瞻性研究。作为对照，研究了20名年龄和性别匹配的健康自愿献血者。我们开发了一种酶联免疫吸附测定法（ELISA）来测量sCD27。SLE患者的sCD27平均水平（±标准差）（48.29±23.86 u）高于对照组（36.13±7.48 u）（P = 0.02）。此外，活动期SLE患者的血清sCD27浓度（58.20±31.06 u）高于缓解期患者（42.77±16.71 u）（P < 0.01）。我们发现SLE患者的sCD27和sCD25血清水平之间存在正相关（r = 0.30，P = 0.01）。未发现与其他临床症状或免疫参数有显著关系。总之，SLE患者的血清sCD27水平升高，且这种升高与疾病活动有关。我们的数据与CD27抗原可能构成疾病活动标志物的假设一致。
