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来自蚕豆的重复DNA序列的初步特征分析。

Preliminary characterization of the repeated DNA sequence from Vicia sativa.

作者信息

Lehmann P, Barnes S

机构信息

Department of Genetics, University of Nottingham, United Kingdom.

出版信息

Acta Biochim Pol. 1990;37(1):21-30.

PMID:2087913
Abstract

We have identified a family of interspersed repeated sequences present in about 40,000 copies in the genomes of Vicia sativa and its near relative Vicia faba. The element vif is at least 6 kb in length, and members of the repeat family display a degree of heterogeneity in restriction pattern. Homologous elements in V. faba are much more heterogeneous than their V. sativa counterparts; however, analysis of five different V. faba lines revealed substantially the same patterns, suggesting that this family was reamplified prior to the divergence of the lines studied.

摘要

我们已经鉴定出一个散布重复序列家族,在蚕豆(Vicia sativa)及其近缘种蚕豆(Vicia faba)的基因组中约有40000个拷贝。vif元件长度至少为6 kb,该重复序列家族的成员在限制性图谱上表现出一定程度的异质性。蚕豆中的同源元件比蚕豆中的同源元件更加异质;然而,对五个不同的蚕豆品系进行分析,结果显示出基本相同的模式,这表明该家族在研究的品系分化之前就已经再次扩增。

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