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[鸟类基因组中度重复序列簇的分子与细胞学分析]

[Molecular and cytologic analysis of clusters of moderate repeats of bird genomes].

作者信息

Tarantul V Z, Gol'tsov V A, Rodionov A V, Chelysheva L A, Khutinaeva M A

出版信息

Mol Biol (Mosk). 1989 Mar-Apr;23(2):481-90.

PMID:2770728
Abstract

Pigeon genome long sequences containing clusters of moderately repeating elements have been cloned. Molecular analysis has shown a dispersed distribution of the repeats in both pigeon and chicken genomes. Within a single cluster, a scrambled distribution of elements belonging to different families of repeats has been shown. Similar repeated sequences have been revealed within clusters. The analysed clusters of repeats are characterized by a limited structural variability in the genomes. In situ hybridization revealed the localization of sequences complementary to the cloned clusters in pigeon and chicken macrochromosomes. Preferential localization has been demonstrated in telomeric and centromeric chromosome regions as well as in the region of R-bands.

摘要

已克隆出含有中度重复元件簇的鸽子基因组长序列。分子分析表明,这些重复序列在鸽子和鸡的基因组中呈分散分布。在单个簇内,已显示属于不同重复序列家族的元件呈不规则分布。在簇内也发现了相似的重复序列。所分析的重复序列簇的特点是在基因组中的结构变异性有限。原位杂交揭示了与克隆簇互补的序列在鸽子和鸡的大染色体中的定位。已证实在端粒和着丝粒染色体区域以及R带区域存在优先定位。

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